Detection of Chlamydia pneumoniae but not Helicobacter pylori in atherosclerotic plaques of aortic aneurysms
Detection of Chlamydia pneumoniae but not Helicobacter pylori in atherosclerotic plaques of aortic aneurysms
复制标题
DOI：
10.1128/jcm.34.11.2766-2769.1996
复制
发表时间：
1996-11-01
影响因子：
9.4
通讯作者：
Allegra, L
中科院分区：
文献类型：
--
作者：
Blasi, F;Denti, F;Allegra, L
最近的报道表明肺炎衣原体和幽门螺杆菌与动脉粥样硬化之间存在联系。我们研究了51例接受腹主动脉瘤手术的患者（平均年龄68.3岁）。我们对每一位患者进行了微量免疫荧光试验，检测抗C抗体的免疫球蛋白G（IgG）、伊加和IgM。肺炎特异性抗原TW-183。抗艾滋通过ETA-G试验测定幽门螺杆菌抗体。将每个主动脉瘤手术标本分成多个0.3 cm（2）的切片，并在-20 ℃下冷冻。每个动脉瘤的两个样本用于两组C的巢式PCR。pneumoniae和两组H. pylori特异性引物，用含有20 mM Tris-HCl、Tween 20-Nonidet P-40（各0.5% [vol/vol]）和100 μ g蛋白酶K/ml，在60 ℃温育1小时，在98 ℃温育10分钟，用苯酚-氯仿-异戊醇提取DNA两次，用乙酸钠-乙醇按标准方法沉淀，41例患者血清C.肺炎克雷伯氏菌有既往感染史者32例（IgG < 512者16例，伊加< 256者32例），高滴度者9例（IgG> 512者26例）。在这些患者中，23例具有血清学既往感染模式，2例具有急性再感染模式，1例为血清阴性。51例患者中有47例血清H.幽门。在所有病例中，PCR均未显示H。pylori的存在，这项研究提供了一个可能的C. pneumoniae参与主动脉瘤的发病机制，以及该药物与动脉粥样硬化之间相关性的额外证据。相反，尽管H。pylori血清阳性率，我们的结果倾向于排除H.幽门螺杆菌在动脉粥样硬化中的作用
Recent reports suggest an association between Chlamydia pneumoniae and Helicobacter pylori bacteria and atherosclerosis. We studied 51 patients (mean age, 68.3 years) who underwent abdominal aortic aneurysm surgery. For each patient we performed a microimmunofluorescence test for immunoglobulin G (IgG), IgA, and IgM antibodies to C. pneumoniae specific antigen (TW-183). Anti-H. pylori antibodies were determined by means of an ETA-G test. Each aortic aneurysm surgical specimen was sampled into multiple sections of 0.3 cm(2) each and frozen at -20 degrees C, Two samples of each aneurysm were used for a nested PCR with two sets of C. pneumoniae and two sets of H. pylori specific primers, Specimens were treated with a solution containing 20 mM Tris-HCl, Tween 20-Nonidet P-40 (0.5% [vol/vol] each), and 100 mu g of proteinase K per ml and incubated at 60 degrees C for 1 h and at 98 degrees C for 10 min, DNA was extracted twice with phenol-chloroform-isoamylic alcohol and precipitated with sodium acetate-ethanol by standard methods, Forty-one patients were seropositive for C. pneumoniae with past-infection patterns in 32 patients (16 less than or equal to IgG < 512; 32 less than or equal to IgA < 256) and high antibody titers in 9 patients (IgG greater than or equal to 512), In 26 of 51 patients, C. pneumoniae DNA was detected in aortic aneurysm plaque specimens, Of these patients, 23 had a serologic past-infection pattern, 2 had an acute reinfection pattern, and 1 was seronegative. Forty-seven of 51 patients were seropositive for H. pylori. In all cases PCR showed no evidence of H. pylori presence in plaque specimens, This study provides data on a possible C. pneumoniae involvement in the pathogenesis of aortic aneurysm and additional evidence for an association between this agent and atherosclerosis. Conversely, notwithstanding a high H. pylori seroprevalence observed, our results tend to rule out the possibility of a direct involvement of H. pylori in atherosclerosis.