Controlled release of bone morphogenetic protein (BMP)-2 from nanocomplex incorporated on hydroxyapatite-formed titanium surface
Controlled release of bone morphogenetic protein (BMP)-2 from nanocomplex incorporated on hydroxyapatite-formed titanium surface
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DOI：
10.1016/j.jconrel.2012.04.021
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发表时间：
2012-06-28
影响因子：
10.8
通讯作者：
Han, Dong Keun
中科院分区：
文献类型：
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作者：
Bae, Soon Eon;Choi, Jiyeon;Han, Dong Keun
骨传导性和骨诱导性在新骨形成和最终骨再生中同样非常重要。本研究的目的是在钛（Ti）表面创造一个不仅具有骨传导性而且具有骨诱导性的环境。为此，利用 BMP-2 和硫酸软骨素 (CS) 之间的离子相互作用制备了骨形态发生蛋白 2 (BMP-2) 纳米复合物 (NC)。同时，对Ti进行化学处理，然后在模拟体液(SBF)中浸泡，将样品命名为Ti(C)-羟基磷灰石(HA)。一旦 BMP-2 NC 沉淀在 Ti(C)-HA 表面，并添加钙/磷酸盐溶液，最终产物即形成 Ti(C)-HA-BMP-2。 NC 的尺寸范围为 150 至 250 nm，CS 的量对确定 NC 尺寸和 zeta 电位有影响。通过 SEM 观察，发现 Ti 表面很好地覆盖着结晶磷灰石层，并通过 FTIR 和 NMR 进行了鉴定。固定的 BMP-2 以中等速率释放，持续 4 周，没有 BMP-2 的初始爆发。当小鼠成骨细胞接种在不同的Ti基质上时，与其他组相比，Ti(C)-HA-BMP-2组的细胞增殖更快。使用 BMP-2 NC 后，骨特异性标记物、骨钙素和 I 型胶原蛋白的基因表达显着上调。碱性磷酸酶活性的测定也有同样的结果，且差异有统计学意义。这项研究表明，BMP-2 NC 的递送系统能够有效地将 BMP-2 固定在磷灰石涂层的钛表面上，并且钛表面可以被修改为骨诱导和骨传导的环境。 (C) 2012 Elsevier B.V. 保留所有权利。
Both osteoconductivity and osteoinductivity are equally very important aspects in a new bone formation and ultimately for bone regeneration. The purpose of this study was to create an environment, not only osteoconductive but also osteoinductive on titanium (Ti) surface. To do this bone morphogenetic protein-2 (BMP-2) nanocomplex (NC) was fabricated by using an ionic interaction between BMP-2 and chondroitin sulfate (CS). Meanwhile, Ti was chemically treated, then subjected to soaking in simulated body fluid (SBF), naming the sample Ti(C)-hydroxyapatite (HA). Once the BMP-2 NC was precipitated on the Ti(C)-HA surface, along with the addition of calcium/phosphate solution, the final product was formed as Ti(C)-HA-BMP-2. The size of NC was ranged from 150 to 250 nm and the amount of CS was influential in determining both NC size and zeta potential. From the SEM observation, Ti surface was found nicely covered with the crystallized apatite layer that was identified using FTIR and NMR. The immobilized BMP-2 was released in a moderate rate for 4 weeks, without an initial burst of BMP-2. When mouse osteoblast cells were seeded on different Ti substrates, cell proliferation was faster in the Ti(C)-HA-BMP-2 group, as compared to other groups. The gene expression of bone-specific markers, osteocalcin and type I collagen, was significantly upregulated with the use of BMP-2 NC. The same result was witnessed in the measurement of alkaline phosphatase activity, in which the difference was statistically significant. This study demonstrated that the delivery system of BMP-2 NC was effective in holding BMP-2 on the apatite-coated Ti surface and that the Ti surface could be modified into the environment osteoinductive as well as osteoconductive. (C) 2012 Elsevier B.V. All rights reserved.