Efficient isolation of CD8α positive T cells from postnatal mice using a combined MACS approach
Efficient isolation of CD8α positive T cells from postnatal mice using a combined MACS approach
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DOI：
10.1016/j.jim.2014.02.006
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发表时间：
2014-04-01
影响因子：
2.2
通讯作者：
Braeuer, Anja U.
中科院分区：
文献类型：
--
作者：
Huebner, Anastasia;Derkow, Katja;Braeuer, Anja U.
磁激活细胞分选（MACS）是一种常用的细胞分离技术。Miltenyi Biotec开发了一种有效的MACS方案，用于分离CD8 α（+）。成年小鼠脾脏T细胞。然而，由于CD8 α（+）的百分比较低。出生后小鼠脾脏中的T细胞，CD8 α（+）的MACS分离。T细胞导致低纯度。我们的研究说明了如何通过在CD8 α（+）T细胞的阳性选择之前进行CD45 R（+）B细胞去除来解决这个问题。当低频率的CD8 α（+）T细胞存在于也聚集有CD45 R（+）B细胞的组织中时，可以使用该方案，并且下游应用需要高纯度。(C)2014爱思唯尔有限公司版权所有。
Magnetic activated cell sorting (MACS) is a commonly used cell separation technique. Miltenyi Biotec has developed an efficient MACS protocol for isolation of CD8 alpha(+). T cells from adult mice spleen. However, due to a low percentage of CD8 alpha(+). T cells in spleen of postnatal mice, MACS isolation of CD8 alpha(+). T cells results in a low purity. Our study illustrates how this problem can be solved by performing a CD45R(+) B cell depletion prior to a positive selection of CD8 alpha(+) T cells. This protocol can be used when low frequencies of CD8 alpha(+) T cells are present in a tissue also populated with CD45R(+) B cells, and a high purity is required for downstream applications. (C) 2014 Elsevier B.V. All rights reserved.