Electrical stimulation of cervical vagal afferents. I. Central relays for modulation of spinal nociceptive transmission.
Electrical stimulation of cervical vagal afferents. I. Central relays for modulation of spinal nociceptive transmission.
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电刺激颈部迷走神经传入。
DOI：
10.1152/jn.1990.64.4.1098
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发表时间：
1990
影响因子：
2.5
通讯作者：
Gebhart,GF
中科院分区：
文献类型：
--
作者：
Ren,K;Randich,A;Gebhart,GF
1. 采用非选择性、可逆局部麻醉或躯体选择性、不可逆神经毒性损伤对50℃皮肤加热脊髓背角神经元迷走神经传入调节的棘上接力进行了研究。80只经戊巴比妥麻醉的瘫痪大鼠腰椎背角分离出85个神经元。所有被研究的神经元在同侧后爪足底表面的无毛皮肤上都有接受野，并且对低强度和高强度的机械刺激以及有害的热刺激都有反应。2. 建立了迷走神经传入刺激（VAS）对皮肤加热神经元反应的强度依赖性调节。低强度VAS促进了40个单位的反应，高强度VAS抑制了40个单位的反应。另外36个单位仅受VAS抑制，4个单位仅受促进。3. 通过双侧微量注射利多卡因（4%，0.5微升）局部麻醉脑桥背外侧，观察该区域对vas产生的脊柱调节的贡献。双侧向蓝斑/蓝斑下（LC/SC）腹侧微量注射利多卡因，可将vas产生的单位热反应抑制率从对照组的63%显著降低至89%，并消除vas产生的促进作用。脊髓背角神经元双侧微量注射利多卡因对瓦斯产生的脊髓背角神经元调节无显著影响。4. 将Ibotenic酸（10微克，0.5微升）微注射到脑桥背外侧，以确定该区域的细胞体对vas产生的脊髓调节的相对贡献。在迷走神经刺激的同侧LC/SC中单侧微量注射伊博tenic酸对vas产生的抑制作用没有显著影响，但显著减弱了vas产生的单位热反应的促进作用。双侧微量注射伊博腾酸显著降低了瓦斯产生的单位对热反应的抑制，从对照组的48%降至94%。5. 局部麻醉内侧前腹侧髓质（RVM），主要是中缝大核（NRM），显著降低了vas产生的单位热反应抑制率，从对照组的55%降至87%，但对vas产生的促进作用没有显著影响。向RVM中微量注射伊博滕酸也显著降低了瓦斯产生的对热的单位反应的抑制。将利多卡因显微注射到NRM背侧区域、中缝苍白核或橄榄核后，未发现vas产生的脊髓调节有明显变化。（摘要删节为400字）
1. Supraspinal relays for vagal afferent modulation of responses of spinal dorsal horn neurons to 50 degrees C heating of the skin were examined by the use of nonselective, reversible local anesthesia or soma-selective, irreversible neurotoxic damage of neural tissue. Eighty-five neurons were isolated in the lumbar spinal dorsal horn of 80 pentobarbital-anesthetized, paralyzed rats. All neurons studied had receptive fields on the glabrous skin of the plantar surface of the ipsilateral hind paw and responded to mechanical stimuli of both low and high intensity as well as noxious thermal stimulation. 2. Intensity-dependent modulation by vagal afferent stimulation (VAS) of neuronal responses to heating of the skin was established. Responses of 40 units were facilitated by low and inhibited by greater intensities of VAS. Another 36 units were only inhibited by VAS, and four were only facilitated. 3. Local anesthesia of the dorsolateral pons by bilateral microinjections of lidocaine (4%, 0.5 microliter) were made to examine the contribution of this area to VAS-produced spinal modulation. The microinjection of lidocaine bilaterally into the ventral locus coeruleus/subcoeruleus (LC/SC) reversibly and significantly attenuated VAS-produced inhibition of unit responses to heat from 63 to 89% of control and abolished VAS-produced facilitation. The microinjection of lidocaine bilaterally into the dorsal LC had no significant effect on VAS-produced modulation of spinal dorsal horn neurons. 4. Ibotenic acid (10 micrograms, 0.5 microliter) was microinjected into the dorsolateral pons to determine the relative contributions of cell bodies in this area to VAS-produced spinal modulation. Unilateral microinjection of ibotenic acid into the LC/SC ipsilateral to the vagus nerve stimulated had no significant effect on VAS-produced inhibition but significantly attenuated VAS-produced facilitation of unit responses to heat. Bilateral microinjections of ibotenic acid significantly attenuated VAS-produced inhibition of unit responses to heat from 48 to 94% of control. 5. Local anesthesia of the medial rostroventral medulla (RVM), primarily the nucleus raphe magnus (NRM), significantly attenuated VAS-produced inhibition of unit responses to heat from 55 to 87% of control but had no significant effect on VAS-produced facilitation. Microinjection of ibotenic acid into the RVM also significantly reduced VAS-produced inhibition of unit responses to heat. No significant change in VAS-produced spinal modulation was found after lidocaine microinjection into areas dorsal to the NRM, the nucleus raphe pallidus, or the olivary nucleus.(ABSTRACT TRUNCATED AT 400 WORDS)