Light-mediated control of DNA transcription in yeast.
Light-mediated control of DNA transcription in yeast.
复制标题
DOI：
10.1016/j.ymeth.2012.08.004
复制
发表时间：
2012-12
期刊：
影响因子：
4.8
通讯作者：
Tucker, Chandra L.
中科院分区：
文献类型：
--
作者：
Hughes, Robert M.;Bolger, Steven;Tapadia, Hersh;Tucker, Chandra L.
存在多种用于酵母中DNA转录的诱导型控制的方法。这些包括使用天然酵母启动子或调节元件，其响应于小分子如半乳糖、甲硫氨酸和铜，或允许通过正交小分子如雌激素调节的工程化系统。虽然化学调控系统易于使用并且可以产生高水平的蛋白质表达，但它们通常对蛋白质水平提供不精确的控制。此外，化学调控系统可以影响酵母中的许多其他蛋白质和途径，激活信号通路或生理反应。在这里，我们描述了几种方法，光介导的控制DNA转录在体内酵母。我们描述了使用红光和光敏色素依赖性系统来诱导GAL 1启动子控制下的基因转录的方法，以及使用蓝光/隐花色素依赖性系统来控制GAL 1启动子或莱克萨操纵子控制下的基因转录的方法。光是剂量依赖性的，应用便宜，容易递送，并且不干扰细胞途径，因此具有优于化学系统的显著优势。
A variety of methods exist for inducible control of DNA transcription in yeast. These include the use of native yeast promoters or regulatory elements that are responsive to small molecules such as galactose, methionine, and copper, or engineered systems that allow regulation by orthogonal small molecules such as estrogen. While chemically regulated systems are easy to use and can yield high levels of protein expression, they often provide imprecise control over protein levels. Moreover, chemically regulated systems can affect many other proteins and pathways in yeast, activating signaling pathways or physiological responses. Here, we describe several methods for light mediated control of DNA transcription in vivo in yeast. We describe methodology for using a red light and phytochrome dependent system to induce transcription of genes under GAL1 promoter control, as well as blue light / cryptochrome dependent systems to control transcription of genes under GAL1 promoter or LexA operator control. Light is dose dependent, inexpensive to apply, easily delivered, and does not interfere with cellular pathways, and thus has significant advantages over chemical systems.
登录
查看更多内容
影响因子：
48
作者：
Kennedy, Matthew J.;Hughes, Robert M.;Peteya, Leslie A.;Schwartz, Joel W.;Ehlers, Michael D.;Tucker, Chandra L.
通讯作者：
Tucker, Chandra L.
影响因子：
46.9
作者：
Yazawa, Masayuki;Sadaghiani, Amir M.;Dolmetsch, Ricardo E.
通讯作者：
Dolmetsch, Ricardo E.
影响因子：
56.9
作者：
Liu, Hongtao;Yu, Xuhong;Lin, Chentao
通讯作者：
Lin, Chentao
DOI：
10.1016/j.bbamcr.2010.12.004
发表时间：
2011-02-01
影响因子：
5.1
作者：
Bodvard, Kristofer;Wrangborg, David;Kall, Mikael
通讯作者：
Kall, Mikael
影响因子：
64.8
作者：
Ni, M;Tepperman, JM;Quail, PH
通讯作者：
Quail, PH