Knockdown of Long Noncoding RNA FTX Inhibits Proliferation, Migration, and Invasion in Renal Cell Carcinoma Cells.
Knockdown of Long Noncoding RNA FTX Inhibits Proliferation, Migration, and Invasion in Renal Cell Carcinoma Cells.
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DOI：
10.3727/096504016x14719078133203
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发表时间：
2017-01-26
影响因子：
3.1
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
He X;Sun F;Guo F;Wang K;Gao Y;Feng Y;Song B;Li W;Li Y
肾细胞癌（RCC）是世界上最常见的肾癌之一。虽然在过去几十年中取得了很大进展，但其发病率和致死率仍在增加。长链非编码rna （lncRNAs）在肿瘤发生中发挥着重要作用。本研究旨在探讨lncRNA FTX在RCC细胞增殖和转移中的详细作用。我们的研究结果表明，FTX在临床RCC组织和培养的RCC细胞中的转录水平均显著上调，并与RCC患者的多个临床参数相关，包括家族状况、肿瘤大小、淋巴转移和TNM分期。通过细胞增殖试验、集落形成试验和细胞周期试验，我们证实了在A498和ACHIN细胞中，特异性shrna敲低FTX可抑制细胞增殖率、集落形成能力，并在G0/G1期阻滞细胞周期。在Transwell实验和伤口愈合实验中，FTX缺失也抑制了细胞的迁移和侵袭。这些数据提示了FTX在RCC中的促癌潜能，使其成为临床RCC诊断和治疗的潜在治疗靶点。
Renal cell carcinoma (RCC) is one of the most common kidney cancers worldwide. Although great progressions have been made in the past decades, its morbidity and lethality remain increasing. Long noncoding RNAs (lncRNAs) are demonstrated to play significant roles in the tumorigenesis. This study aimed to investigate the detailed roles of lncRNA FTX in RCC cell proliferation and metastasis. Our results showed that the transcript levels of FTX in both clinical RCC tissues and the cultured RCC cells were significantly upregulated and associated with multiple clinical parameters of RCC patients, including familial status, tumor sizes, lymphatic metastasis, and TNM stages. With cell proliferation assays, colony formation assays, and cell cycle assays, we testified that knockdown of FTX in A498 and ACHIN cells with specific shRNAs inhibited cell proliferation rate, colony formation ability, and arrested cell cycle in the G0/G1 phase. FTX depletion also suppressed cell migration and invasion with Transwell assays and wound-healing assays. These data indicated the pro-oncogenic potential of FTX in RCC, which makes it a latent therapeutic target of RCC diagnosis and treatment in the clinic.