Comparative in Vivo Confocal Microscopical Study of the Cornea Anatomy of Different Laboratory Animals
Comparative in Vivo Confocal Microscopical Study of the Cornea Anatomy of Different Laboratory Animals
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DOI：
10.3109/02713683.2010.513796
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发表时间：
2010-12-01
影响因子：
2
通讯作者：
Stachs, Oliver
中科院分区：
文献类型：
--
作者：
Reichard, Maria;Hovakimyan, Marine;Stachs, Oliver
目的：材料和方法：博美粗毛羊1只，Beagle犬5只，挪威短毛猫1只，家猫2只，新西兰白色兔20只，Wistar大鼠6只，Balb/c小鼠10只。使用配备罗斯托克角膜模块的海德堡视网膜断层扫描仪进行双侧检查。活体角膜层的形态可视化，并在物种之间进行比较。中央角膜厚度，密度的keratocytes，和内皮cells进行了quantited.Results：上皮多层动物类型之间的形态表现出相似性，显示三个明显区分层：浅表，中间，和基底。基底下神经纤维在基底细胞下呈高反射结构。在所有物种中均证实了亚基纤维，但密度在物种之间存在差异。在绵羊中可见明显的Bowman膜。然而，在所有其他物种中，在基底上皮细胞和前基质之间可以看到一层薄的无细胞层，上面覆盖着神经纤维。除小鼠外，所有物种的角膜细胞核均可显示，小鼠未检测到细胞核，仅检测到类似角膜细胞胞体的反射结构。总的来说，角膜基质细胞核的密度在前基质中显著高于后基质中。除内皮细胞密度外，除绵羊外，所有物种的内皮细胞形态非常相似。内皮细胞呈多边形结构，胞浆明亮，边界暗。在绵羊中，内皮细胞的外观是非常差的，因为厚的超反射德斯密氏membrane.Conclusions：本研究将有助于研究人员考虑适当的动物实验模型，根据调查的重点。体内CLSM可用于表征活体角膜随时间的变化，从而减少动物实验的数量。
Purpose: The aim of the present study was to analyze and compare in vivo morphology of healthy cornea of six different laboratory animals.Matherials and Methods: One Pomeranian Coarsewool sheep, 5 Beagle dogs, 1 Norwegian and 2 Domestic Short-haired cats, 20 New Zealand White rabbits, 6 Wistar rats, and 10 Balb/c mice were included. The examination was performed bilaterally, using Heidelberg Retina Tomograph equipped with Rostock Cornea Module. The morphology of living corneal layers was visualized and compared between species. The central corneal thickness, density of keratocytes, and endothelial cells were quantified.Results: The epithelial multilayer showed a similarity in morphology between animal types, displaying three clearly distinguishable layers: superficial, intermediate, and basal. Subbasal nerve fibers were displayed as hyperreflective structures underneath basal cells. The subbasal fibers were confirmed in all species, however, the density varied between species. A pronounced Bowman's membrane was visualized in sheep. In all other species, however, a thin acellular layer with overlying nerve fibers could be seen between basal epithelial cells and anterior stroma. The keratocytes nuclei could be demonstrated in all species except for mice, where no nuclei but only reflective structures resembling keratocytes cell bodies were detected. Overall, the density of keratocytes nuclei was significantly higher in the anterior than in the posterior stroma. Besides endothelial cells density, the endothelial cells morphology was very similar among all species, except for sheep. The endothelial cells were displayed as polygonal structures with bright cytoplasm and dark borders. In sheep, the appearance of the endothelium was very poor because of a thick hyperreflective Descemet's membrane.Conclusions: The present study will help researchers consider appropriate models for animal experiments, depending on focus of investigation. In vivo CLSM can be used for the characterization of the living cornea over time, thus, reducing the number of animal experiments.