Decreased expressions of c-myc and H-ras oncogenes in vitamin E succinate induced morphologically differentiated murine B-16 melanoma cells in culture.
Decreased expressions of c-myc and H-ras oncogenes in vitamin E succinate induced morphologically differentiated murine B-16 melanoma cells in culture.
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DOI：
10.1139/o90-185
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发表时间：
1990-11
期刊：
影响因子：
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通讯作者：
K. Prasad;R. Cohrs;O. K. Sharma
中科院分区：
文献类型：
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作者：
K. Prasad;R. Cohrs;O. K. Sharma
用浓度为11.3和15.1 μ M的d-α-生育酚琥珀酸酯（维生素E琥珀酸酯）处理培养物中的B-16黑色素瘤细胞可抑制生长并诱导培养物中的细胞分化。维生素E琥珀酸酯处理降低了黑色素瘤细胞中c-myc和H-ras特异性mRNA的水平。维生素视黄酸和非维生素药物R 020 -1724（4-（3-丁氧基-4-甲氧基苄基）-2-咪唑啉酮）（一种环核苷酸磷酸二酯酶抑制剂（0.72 mM））和丁酸钠（1 mM）也获得了类似的结果，它们诱导了培养物中黑素瘤细胞的分化和（或）抑制其生长。c-myc mRNA的抑制程度大于H-ras mRNA。这些结果表明，维生素E琥珀酸酯诱导的c-myc和H-ras mRNA水平的降低与培养物中黑素瘤细胞的生长抑制有关。
Treatment of B-16 melanoma cells in culture with d-alpha-tocopheryl succinate (vitamin E succinate) at concentrations of 11.3 and 15.1 microM inhibited growth and induced cell differentiation in culture. Vitamin E succinate treatment decreased the levels of c-myc and H-ras specific mRNAs in melanoma cells. Similar results were obtained by the vitamin retinoic acid and the nonvitamin agents R020-1724 (4-(3-butoxy-4-methoxybenzyl)-2-imidazolidinone), an inhibitor of cyclic nucleotide phosphodiesterase (0.72 mM), and sodium butyrate (1 mM), which induced differentiation and (or) inhibited growth of melanoma cells in culture. The extent of inhibition of c-myc mRNA was greater than that of H-ras mRNA. These results indicate that vitamin E succinate induced reduction of the levels of c-myc and H-ras mRNAs is related to growth inhibition of melanoma cells in culture.