Human amelogenin up-regulates osteogenic gene expression in human bone marrow stroma cells
Human amelogenin up-regulates osteogenic gene expression in human bone marrow stroma cells
复制标题
人釉原蛋白上调人骨髓基质细胞中的成骨基因表达
DOI：
10.1016/j.bbrc.2011.04.042
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发表时间：
2011-05-13
影响因子：
3.1
通讯作者：
Chen Lan
中科院分区：
文献类型：
--
作者：
Hu Jingchao;Shu Rong;Chen Lan
釉质基质蛋白提取物用于再生牙周组织。釉原蛋白是最丰富的釉质蛋白，在这些组织的再生中起着重要的作用。然而，釉原蛋白促进牙周再生的分子机制仍不清楚。利用转导慢病毒编码人釉原蛋白(HAM)的原代人骨髓基质细胞(HBMSCs)进行全基因组表达谱分析，以分析HAM转导对成骨分化相关基因调控的影响。结果表明，BMP-2、BMP-6、OPN和VEGFC表达上调。这些结果提示HAM可能是调节hBMSCs成骨分化的关键因素。皇冠版权所有(C)2011由爱思唯尔公司出版。保留所有权利。
Extracts of enamel matrix proteins are used to regenerate periodontal tissues. Amelogenin, the most abundant enamel protein, plays an important role in the regeneration of these tissues. However, the molecular mechanisms by which amelogenin contributes to periodontal regeneration remain unknown. Using primary human bone marrow stroma cells (hBMSCs) transduced with lentivirus encoding human amelogenin (hAm), we performed genome-wide expression profiling to analyze the effects of hAm transduction on the regulation of genes involved in osteogenic differentiation. Our results revealed that BMP-2, BMP-6, OPN and VEGFC were up-regulated. These results suggest that hAm may be a key element in regulating hBMSCs osteogenic differentiation. Crown Copyright (C) 2011 Published by Elsevier Inc. All rights reserved.