Identification of cytochrome P450 1A1 in human brain.
Identification of cytochrome P450 1A1 in human brain.
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DOI：
10.1006/bbrc.1998.8171
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发表时间：
1998-02
影响因子：
3.1
通讯作者：
C. Yun;H. Park;S. J. Kim;H. K. Kim
中科院分区：
文献类型：
--
作者：
C. Yun;H. Park;S. J. Kim;H. K. Kim
我们通过聚合酶链式反应 (PCR) 分析分离了源自人脑 P450 1A1 的 cDNA 片段。它们的大小与我们根据报道的人乳腺癌细胞系 MCF-7 的 P450 1A1 mRNA 序列所预期的相符。为了确认正确的 PCR 产物，对它们进行了亚克隆并确定了它们的核苷酸序列。这一结果表明PCR产物实际上来源于人脑中的P450 1A1 mRNA。我们首次证明人脑含有 P450 1A1 依赖性代谢系统，这对于理解内源性和外源性化合物的代谢似乎很重要。
We have isolated cDNA fragments that were originated from P450 1A1 in human brain by polymerase chain reaction (PCR) analysis. Their size matched up to what we expected based on the reported P450 1A1 mRNA sequence of human breast carcinoma cell line MCF-7. To confirm proper PCR products, they were subcloned and their nucleotide sequences were determined. This result showed that PCR products were actually derived from P450 1A1 mRNA in human brain. We demonstrated for the first time that human brain contains a P450 1A1-dependent metabolism system which seems to be important for understanding the metabolism of endogenous and exogenous compounds.