Heterotrimeric G-Protein Interactions Are Conserved Despite Regulatory Element Loss in Some Plants
Heterotrimeric G-Protein Interactions Are Conserved Despite Regulatory Element Loss in Some Plants
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DOI：
10.1104/pp.20.01309
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发表时间：
2020-12-01
期刊：
影响因子：
7.4
通讯作者：
Pandey, Sona
中科院分区：
文献类型：
--
作者：
Bhatnagar, Nikita;Pandey, Sona
G蛋白α亚基及其调节蛋白RGS之间的功能网络在植物中是保守的，尽管在许多单子叶植物中不存在RGS，但它们相应的G α蛋白保留了在植物中被非天然RGS失活的能力。异源三聚体G蛋白是植物多种信号传导和发育途径的关键调节剂，并调节许多农艺性状，包括结构和谷物产量。G蛋白信号调节蛋白（Regulator of G-protein signaling，RGS）是G蛋白网络的一个组成部分，但在许多单子叶植物中丢失。为了评估特定植物中RGS的丢失是否导致G蛋白网络的改变以及RGS功能在对比单子叶植物中保守的程度，我们探索了二穗短柄草和狗尾草中G蛋白依赖的发育途径，分别代表没有或具有天然RGS的物种。在这两个物种中，基于microRNA的G α人工抑制导致了相似的表型。此外，在B.二穗子导致与BdG α抑制相似的表型。RGS过表达的这种效果取决于其使G α失活的能力，因为生物化学上无活性的变体蛋白的过表达导致植物与野生型难以区分。B的比较转录组分析。具有抑制水平的G α或RGS过表达的二穗草植物显示差异调节基因的显著重叠，证实了表型数据。这些结果表明，尽管在许多单子叶植物中RGS的损失，G-蛋白功能网络得以维持，并且G α蛋白保留了它们被RGS失活的能力。
The functional networks between the G-protein alpha -subunit and its regulatory protein, RGS, are conserved in plants, and despite the absence of RGS in many monocots, their corresponding G alpha proteins have retained the ability to be deactivated by nonnative RGS in planta. Heterotrimeric G-proteins are key modulators of multiple signaling and development pathways in plants and regulate many agronomic traits, including architecture and grain yield. Regulator of G-protein signaling (RGS) proteins are an integral part of the G-protein networks; however, these are lost in many monocots. To assess if the loss of RGS in specific plants has resulted in altered G-protein networks and the extent to which RGS function is conserved across contrasting monocots, we explored G-protein-dependent developmental pathways in Brachypodium distachyon and Setaria viridis, representing species without or with a native RGS, respectively. Artificial microRNA-based suppression of G alpha in both species resulted in similar phenotypes. Moreover, overexpression of Setaria italica RGS in B. distachyon resulted in phenotypes similar to the suppression of BdG alpha. This effect of RGS overexpression depended on its ability to deactivate G alpha, as overexpression of a biochemically inactive variant protein resulted in plants indistinguishable from the wild type. Comparative transcriptome analysis of B. distachyon plants with suppressed levels of G alpha or overexpression of RGS showed significant overlap of differentially regulated genes, corroborating the phenotypic data. These results suggest that despite the loss of RGS in many monocots, the G-protein functional networks are maintained, and G alpha proteins have retained their ability to be deactivated by RGS.