Human mannose-binding lectin preferentially binds to human colon adenocarcinoma cell lines expressing high amount of Lewis A and Lewis B antigens.
Human mannose-binding lectin preferentially binds to human colon adenocarcinoma cell lines expressing high amount of Lewis A and Lewis B antigens.
复制标题
人甘露糖结合凝集素优先结合表达大量 Lewis A 和 Lewis B 抗原的人结肠腺癌细胞系。
DOI：
10.1248/bpb.22.347
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发表时间：
1999
影响因子：
2
通讯作者：
Kojiro Matsumoto
中科院分区：
文献类型：
--
作者：
S. Muto;K. Sakuma;A. Taniguchi;Kojiro Matsumoto
采用流式细胞术分析了人甘露糖结合凝集素（MBL）与人结肠癌细胞株和白血病细胞株的结合情况。在7种结肠癌细胞系（col205、col201和DLD-1）中的3种中观察到MBL结合，但在3种白血病细胞系中均未观察到MBL结合。MBL与这些细胞系的结合是糖特异性的和钙依赖性的，因为它在10 mM EDTA或50 mM甘露糖的存在下几乎完全被抑制。用o连接的糖基化抑制剂benzyl-2-acetamide-2-deoxy- α - galnac （bz - α - galnac）预处理细胞，比用n连接的糖基化抑制剂tunicamycin预处理细胞，MBL与col205细胞的结合更强烈地降低。MBL的结合程度与Lewis A和Lewis B抗原在这些细胞系上的表达密切相关。此外，抗lewis A和抗lewis B抗体可抑制MBL与col205细胞的结合。这些结果表明MBL可以通过其Lewis A和Lewis B片段与某些人结肠癌细胞系结合。
The binding of human mannose-binding lectin (MBL) to human colon adenocarcinoma cell lines and leukemia cell lines was analyzed by flow cytometry using specific antibodies against MBL. MBL binding was observed in 3 of 7 colon adenocarcinoma cell lines (Colo205, Colo201 and DLD-1), but not in any of 3 leukemia cell lines tested. The binding of MBL to these cell lines was sugar-specific and calcium-dependent, since it was almost completely inhibited in the presence of 10 mM EDTA or 50 mM mannose. The MBL binding to Colo205 cells was more strongly reduced by the pretreatment of the cells with an O-linked glycosylation inhibitor, benzyl-2-acetamide-2-deoxy-alpha-galactopyranoside (Bz-alpha-GalNAc), rather than an N-linked glycosylation inhibitor, tunicamycin. The degree of MBL binding was well correlated with the expression of Lewis A and Lewis B antigens on these cell lines. Moreover, MBL binding to Colo205 cells was inhibited by anti-Lewis A and anti-Lewis B antibodies. These results suggest that MBL could bind to some human colon adenocarcinoma cell lines through their Lewis A and Lewis B moieties.