DOSE-RATE EFFECT FOR DNA-DAMAGE INDUCED BY IONIZING-RADIATION IN HUMAN TUMOR-CELLS
DOSE-RATE EFFECT FOR DNA-DAMAGE INDUCED BY IONIZING-RADIATION IN HUMAN TUMOR-CELLS
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DOI：
10.2307/3578771
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发表时间：
1994-04-01
影响因子：
3.4
通讯作者：
MCMILLAN, TJ
中科院分区：
文献类型：
--
作者：
DEALMODOVAR, JMR;BUSH, C;MCMILLAN, TJ
在电离辐射处理的人膀胱癌细胞系RT 112中，研究了剂量率对克隆形成细胞存活和DNA双链断裂（DSB）的影响。细胞存活率在所用剂量率范围内（0.01-1.28戈伊/min）发生显著变化，随着剂量率降低，曲线变得更浅和更直。同样，脉冲场凝胶电泳（PFGE）测量的DSB的数量照射后立即随剂量率而变化。低剂量率照射后可检测到较少的DSB。然而，当照射后允许4小时的修复期时，在所有剂量率下处理的细胞表现出大致相同的损伤量。因此，PFGE检测到的未结合DSB的最终水平与不同剂量率下的细胞存活率无关。
The effect of dose rate on clonogenic cell survival and DNA double-strand breaks (DSBs) has been examined in a human bladder carcinoma cell line, RT112, treated with ionizing radiation. Cell survival changed markedly over the range of dose rates used (0.01-1.28 Gy/min) with the curves becoming shallower and straighter as the dose rate was lowered. Similarly, the number of DSBs measured by pulsed-field gel electrophoresis (PFGE) immediately after irradiation varied with dose rate. Fewer DSBs were detectable after low-dose-rate irradiation. However, when a 4-h repair period was allowed after irradiation, cells treated at all dose rates exhibited approximately the same amount of damage. The final level of unrejoined DSBs, as detected by PFGE, therefore does not correlate with cell survival at different dose rates.