Combined Magnetic Tweezers and Micro-mirror Total Internal Reflection Fluorescence Microscope for Single-Molecule Manipulation and Visualization.
Combined Magnetic Tweezers and Micro-mirror Total Internal Reflection Fluorescence Microscope for Single-Molecule Manipulation and Visualization.
复制标题
组合磁镊和微镜全内反射荧光显微镜，用于单分子操作和可视化。
DOI：
10.1007/978-1-4939-7271-5_16
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Neuman,KeirC
中科院分区：
文献类型：
--
作者：
Seol,Yeonee;Neuman,KeirC
Magnetic tweezers is a versatile yet simple single-molecule manipulation technique that has been used to study a broad range of nucleic acids and nucleic acid-based molecular motors. In this chapter, we combine micro-mirror-based total internal reflection microscopy with a magnetic tweezers instrument, permitting simultaneous single-molecule visualization and mechanical manipulation. We provide a simple method to calibrate the evanescent wave penetration depth via supercoiling of DNA with a fluorescent nanodiamond-labeled magnetic bead and a complementary method employing a surface-immobilized fluorescent nanodiamond.
