Measurement of the Compressibility of Cell and Nucleus Based on Acoustofluidic Microdevice
Measurement of the Compressibility of Cell and Nucleus Based on Acoustofluidic Microdevice
复制标题
基于声流控微器件的细胞和细胞核压缩性测量
DOI：
10.3791/64225
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发表时间：
2022-07-01
影响因子：
1.2
通讯作者：
Liu, Yang
中科院分区：
文献类型：
--
作者：
Fu, Qibin;Zhang, Yan;Liu, Yang
细胞力学在肿瘤转移、细胞恶性转化和放射敏感性中起重要作用。在这些过程中，研究细胞的机械性能往往是具有挑战性的。传统的基于接触的测量方法，如压缩或拉伸，容易造成细胞损伤，影响测量精度和后续的细胞培养。粘附状态下的测量也会影响准确性，特别是在照射后，因为电离辐射会使细胞变平并增强粘附。本文介绍了一种基于声流技术的细胞力学测量系统。通过记录细胞在声力作用下的运动轨迹，可以得到细胞的可压缩性，实现悬浮状态下的快速无损测量。本文详细报道了芯片设计、样品制备、轨迹记录、参数提取和分析的协议。利用该方法测量了不同类型肿瘤细胞的可压缩性。通过调节压电陶瓷的谐振频率和微通道的宽度，实现了核的可压缩性的测量。结合免疫荧光实验的分子水平验证，比较药物诱导上皮向间充质转化（EMT）前后细胞的可压缩性。进一步揭示了不同剂量X射线照射后细胞可压缩性的变化。本文提出的细胞力学测量方法具有通用性和灵活性，在科学研究和临床实践中具有广阔的应用前景。
Cell mechanics play an important role in tumor metastasis, malignant transformation of cells, and radiosensitivity. During these processes, studying the mechanical properties of the cells is often challenging. Conventional measurement methods based on contact such as compression or stretching are prone to cause cell damage, affecting measurement accuracy and subsequent cell culture. Measurements in adherent state can also affect accuracy, especially after irradiation since ionizing radiation will flatten cells and enhance adhesion. Here, a cell mechanics measurement system based on acoustofluidic method has been developed. The cell compressibility can be obtained by recording the cell motion trajectory under the action of the acoustic force, which can realize fast and non-destructive measurement in suspended state. This paper reports in detail the protocols for chip design, sample preparation, trajectory recording, parameter extraction and analysis. The compressibility of different types of tumor cells was measured based on this method. Measurement of the compressibility of nucleus was also achieved by adjusting the resonance frequency of the piezoelectric ceramic and the width of the microchannel. Combined with the molecular level verification of immunofluorescence experiments, the cell compressibility before and after druginduced epithelial to mesenchymal transition (EMT) were compared. Further, the change of cell compressibility after X-ray irradiation with different doses was revealed. The cell mechanics measurement method proposed in this paper is universal and flexible and has broad application prospects in scientific research and clinical practice.