Characterization of Organic Acid Metabolism and Expression of Related Genes During Fruit Development of Actinidia eriantha 'Ganmi 6'
Characterization of Organic Acid Metabolism and Expression of Related Genes During Fruit Development of Actinidia eriantha 'Ganmi 6'
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毛花猕猴桃‘甘蜜6号’果实发育过程中有机酸代谢及相关基因表达特征
DOI：
10.3390/plants9030332
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发表时间：
2020-03-01
期刊：
影响因子：
4.5
通讯作者：
Xu, Xiaobiao
中科院分区：
文献类型：
--
作者：
Jiang, Zhiqiang;Huang, Qing;Xu, Xiaobiao
有机酸代谢的研究主要集中在果实上，然而，很少有研究关注毛花猕猴桃果实中高有机酸含量的机制。以11个发育时期采收的‘赣猕6号’果实为材料。采用高效液相色谱（HPLC）系统测定有机酸的成分和含量，检测相关酶的活性，并通过实时定量聚合酶链反应（qRT - PCR）测定基因表达水平。检测到抗坏血酸（AsA）和8种有机酸的成分。这些结果表明，奎尼酸和柠檬酸是‘赣猕6号’果实中的主要有机酸。相关性分析表明，NADP - 奎尼酸脱氢酶（NADP - QDH）、NADP - 莽草酸脱氢酶（NADP - SDH）和细胞液乌头酸酶（Cyt - Aco）可能参与调节有机酸的生物合成。同时，SDH基因可能在调节柠檬酸的积累中起重要作用。在本研究中，NADP - SDH、线粒体乌头酸酶（Mit - Aco）和NAD - 异柠檬酸脱氢酶（NAD - IDH）的活性在转录水平上受其相应基因的调控。柠檬酸合酶（CS）的活性可能受翻译后修饰的影响。我们的研究结果为毛花猕猴桃果实有机酸代谢特征提供了新的见解。
Studies on organic acid metabolism have been mainly concentrated on the fruit, whereas, few have focused on the mechanism of high organic acids content in the fruit of Actinidia eriantha. Fruits of 'Ganmi 6' harvested at eleven developmental periods were used as materials. The components and content of organic acids were determined by high-performance liquid chromatography (HPLC) system, the activities of the related enzyme were detected, and gene expression levels were measured by quantitative real-time PCR (qRT-PCR). Components of ascorbic acid (AsA) and eight kinds of organic acids were detected. These results showed that quinic acid and citric acid were the main organic acids in the fruit of 'Ganmi 6'. Correlation analysis showed that NADP-Quinate dehydrogenase (NADP-QDH), NADP-Shikimate dehydrogenase (NADP-SDH), and Cyt-Aconitase (Cyt-Aco) may be involved in regulating organic acids biosynthesis. Meanwhile, the SDH gene may play an important role in regulating the accumulation of citric acid. In this study, the activities of NADP-SDH, Mit-Aconitase (Mit-Aco), and NAD-Isocitrate dehydrogenase (NAD-IDH) were regulated by their corresponding genes at the transcriptional level. The activity of Citrate synthase (CS) may be affected by post-translational modification. Our results provided new insight into the characteristics of organic acid metabolism in the fruit of A. eriantha.