Photomorphogenesis in the hypogeous fungus Tuber borchii:: isolation and characterization of Tbwc-1, the homologue of the blue-light photoreceptor of Neurospora crassa
Photomorphogenesis in the hypogeous fungus Tuber borchii:: isolation and characterization of Tbwc-1, the homologue of the blue-light photoreceptor of Neurospora crassa
复制标题
DOI：
10.1016/j.fgb.2004.02.004
复制
发表时间：
2004-07-01
影响因子：
3
通讯作者：
Ballario, P
中科院分区：
文献类型：
--
作者：
Ambra, R;Grimaldi, B;Ballario, P
块菌是一组植物共生子囊菌，其下消化道生命周期知之甚少。在这里，我们提出了初步的证据，光对块茎borchii菌丝生长的影响和鉴定和克隆的基因，Tbwc-1，同源的蓝光感光粗糙脉孢菌。蓝光照射T borchii菌落抑制其顶端生长。它也改变了N.粗鲁。在脉孢菌中，这种反应由核光感受器NcWC-1（白色项圈-1）控制，它由传感器结构域（LOV）和转录因子部分组成。我们分离了一个基因（Tbwc-1），其推导的氨基酸序列显示出与NeWC-1的高度相似性和共线性的结构域，除了多聚谷氨酰胺区域。如先前在脉孢菌中发现的，Tbwc-1 mRNA在光控制下，并且其稳态水平在照射后增加。TbWC-1传感器结构域（LOV）的计算机分析支持TbWC-1是光感受器的假设，而在脉孢菌中参与转录激活的两个多聚谷氨酰胺区域的缺失表明这种功能在块茎中可能丢失。(C)2004年爱思唯尔公司All rights reserved.
Truffles form a group of plant-symbiotic Ascomycetes whose hypogeous life cycle is poorly understood. Here we present initial evidence for the influence of light on Tuber borchii mycelial growth and the identification and cloning of a gene, Tbwc-1, homologous to a blue-light photoreceptor of Neurospora crassa. Blue-light irradiation of T borchii colonies inhibits their apical growth. It also alters apical growth in N. crassa. In Neurospora, the response is controlled by a nuclear photoreceptor, NcWC-1 (White Collar-1), which consists of a sensor domain (LOV) and a transcriptional factor moiety. We isolated a gene (Tbwc-1) whose deduced amino acid sequence shows a high similarity and colinearity of domains with NeWC-1, except for the polyglutamine regions. As previously found in Neurospora, Tbwc-1 mRNA is under light control and its steady state level increases upon irradiation. In silico analysis of the TbWC-1 sensor domain (LOV) supports the hypothesis that TbWC-1 is a photoreceptor, while the absence of the two polyglutamine regions involved in transcriptional activation in Neurospora suggests that this function in Tuber could be lost. (C) 2004 Elsevier Inc. All rights reserved.