Cellular Imaging Using Equivalent Cross-Relaxation Rate Technique in Rabbit VX-2 Tumor Model.
Cellular Imaging Using Equivalent Cross-Relaxation Rate Technique in Rabbit VX-2 Tumor Model.
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DOI：
10.4137/cin.s7833
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发表时间：
2011
影响因子：
2
通讯作者：
Kichikawa K
中科院分区：
文献类型：
--
作者：
Nishiofuku H;Matsushima S;Taguchi O;Inaba Y;Yamaura H;Sato Y;Tanaka T;Kichikawa K
等效交叉弛豫率（ECR）成像（ECRI）是一种测量技术，可用于通过MRI定量评价结构组织和细胞密度的变化。本研究的目的是评估兔VX 2肿瘤模型中ECR值与细胞密度之间的相关性。本研究包括5只在股骨肌肉中植入10个VX 2肿瘤的家兔。我们采用了非共振技术，在水共振的低场，采用了7 ppm的单饱和转移脉冲频率。ECR值被定义为不饱和和饱和图像之间的信号损失的百分比。根据ECR值的百分比构建ECR图像。将病理标本分为34个区域，并分为两组：存活组和坏死组。测量ECR值并进行组间比较。然后确定ECR值和细胞密度之间的相关性。存活组的平均ECR值显著高于坏死组（61.2% vs. 35.8%）。受试者工作特征曲线计算的曲线下面积为0.991。回归图显示ECR值与细胞密度之间存在线性关系;相关系数（r）为0.858。在VX 2肿瘤中，ECR值和细胞密度之间有很强的相关性，因此ECRI可能是一种用于准确描绘存活和坏死区域的潜在有用技术。
Equivalent cross-relaxation rate (ECR) imaging (ECRI) is a measurement technique that can be used to quantitatively evaluate changes in structural organization and cellular density by MRI. The aim of this study was to evaluate the correlation between the ECR value and cellular density in the rabbit VX2 tumor model. Five rabbits implanted with 10 VX2 tumors in the femur muscles were included in this study. We adopted the off-resonance technique with a single saturation transfer pulse frequency of 7 ppm downfield from water resonance. The ECR value was defined as the percentage of signal loss between the unsaturated and saturated images. ECR images were constructed based on the percentage of the ECR value. Pathological specimens were divided into 34 areas and classified into two groups: the viable group and the necrotic group. ECR values were measured and compared between groups. The correlation between the ECR value and cellular density was then determined. The mean ECR value was significantly higher in the viable group than in the necrotic group (61.2% vs. 35.8%). The area under the curve that calculated by receiver operating characteristic curve was 0.991 at 7 ppm. The regression graph showed a linear relationship between the ECR value and cellular density; the correlation coefficient (r) was 0.858. There is a strong association between the ECR value and cellular density in VX2 tumors and so ECRI could be a potentially useful technique for accurately depicting viable and necrotic areas.