Origin sites of spontaneous cortical spreading depression migrated during focal cerebral ischemia in rats
Origin sites of spontaneous cortical spreading depression migrated during focal cerebral ischemia in rats
复制标题
大鼠局灶性脑缺血期间自发性皮质扩散抑制的起源部位发生迁移
DOI：
10.1016/j.neulet.2006.05.012
复制
发表时间：
2006-08-07
影响因子：
2.5
通讯作者：
Luo, Qingming
中科院分区：
文献类型：
--
作者：
Chen, Shangbin;Li, Pengcheng;Luo, Qingming
自发性皮质扩散性抑制（CSD）已被发现发生在局灶性脑缺血大鼠的脑半影区，并已被证明可促进梗死的扩大。CSD的电生理记录已被用于监测半影区，但具有固有的低空间分辨率;因此，光学固有信号成像（OISI）被应用于表征永久性左侧大脑中动脉闭塞（MCAO）大鼠在α-氯醛糖/乌拉坦麻醉下的自发CSD波。除了先前报道的MCAO后的自发CSD期间的光学反射率的区域变化，CSD的起源位置很容易确定使用OISI与高分辨率的优势，在本研究中。这些原点（n = 82）动态位于同侧半球皮质中：有时在顶叶皮质中6 mm x 8 mm观察区域之外（n = 19，23%），有时在内部（n = 63，77%）。数据显示出朝向内侧皮质的总体趋势（0.40 +/- 0.15 mm/CSD）。由于大鼠大脑的外侧皮质在4小时闭塞后用2%2，3，5-氯化三苯基四氮唑（TTC）染色证明是梗塞的，因此起源部位的迁移暗示梗塞区域的生长。因此，使用OISI确定自发性CSD的起源将有助于卒中的继续研究。(c)2006爱思唯尔爱尔兰有限公司保留所有权利。
Spontaneous cortical spreading depression (CSD) has been found to occur in the penumbral zone of the brain in rats with focal cerebral ischemia, and has been shown to promote expansion of infarction. Electrophysiological recording of CSD has been used for monitoring the penumbral zone, but with an inherently low spatial resolution; consequently, optical intrinsic signal imaging (OISI) was applied to characterize the spontaneous CSD waves following permanent left middle cerebral artery occlusion (MCAO) in rats under alpha-chloralose/urethane anesthesia. Besides the previous report about the regional variation of optical reflectance during spontaneous CSD following MCAO, the origin site of CSD was easily determined using OISI with the benefit of high resolution in the present study. Those origin points (n = 82) were dynamically located in the ipsilateral hemisphere cortex: sometimes outside of the 6 mm x 8 mm observation area in the parietal cortex (n = 19, 23%), and sometimes inside (n = 63, 77%). The data showed a general trend towards the medial cortex (0.40 +/- 0.15 mm per CSD). Because the lateral cortex of the rat brain proved to be infarcted with 2% 2,3,5-triphenyltetrazolium chloride (TTC) staining after 4 h occlusion, the migration of the origin sites implied a growth of the infarcted area. Hence, the determination of origins of spontaneous CSD using OISI would contribute to the continued study of stroke. (c) 2006 Elsevier Ireland Ltd. All rights reserved.