CD marker expression profiles of human embryonic stem cells and their neural derivatives, determined using flow-cytometric analysis, reveal a novel CD marker for exclusion of pluripotent stem cells
CD marker expression profiles of human embryonic stem cells and their neural derivatives, determined using flow-cytometric analysis, reveal a novel CD marker for exclusion of pluripotent stem cells
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DOI：
10.1016/j.scr.2008.08.001
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发表时间：
2009-03-01
影响因子：
1.2
通讯作者：
Narkilahti, Susanna
中科院分区：
文献类型：
--
作者：
Sundberg, Maria;Jansson, Linda;Narkilahti, Susanna
人胚胎干细胞（human embryonic stem cells，hESC）是一种多能性细胞，可以分化为神经细胞系。这些神经群体通常是异质的，并且可以包含能够在细胞移植物中产生畸胎瘤的未分化多能细胞。hESC及其神经衍生物的表面蛋白质谱的表征对于确定可用于从神经群体中排除未分化细胞的特异性标志物是重要的。在这项研究中，我们分析了分化（CD）标记物的表达谱的7个未分化的人胚胎干细胞用流式细胞仪分析，并比较其配置文件的神经衍生物。干细胞和祖细胞标志物CD133和上皮粘附分子标志物CD326在未分化的hESC中更高表达，而神经标志物CD56（NCAM）和神经前体标志物（趋化因子受体）CD184在hESC衍生的神经细胞中更高表达。CD326表达水平在所有未分化的hESC系中始终高于神经细胞衍生物。此外，CD326阳性的hESC在SCID小鼠睾丸中产生畸胎瘤，而CD362阴性的神经细胞群则没有。因此，CD326可以作为未分化的hESC的新标记物，以在移植前将未分化的hESC从分化的神经细胞群中排除。(C)2008 Elsevier B.V.保留所有权利。
Human embryonic stem cells (hESCs) are pluripotent cells that can differentiate into neural cell lineages. These neural populations are usually heterogeneous and can contain undifferentiated pluripotent cells that are capable of producing teratomas in cell grafts. The characterization of surface protein profiles of hESCs and their neural derivatives is important to determine the specific markers that can be used to exclude undifferentiated cells from neural populations. In this study, we analyzed the cluster of differentiation (CD) marker expression profiles of seven undifferentiated hESC tines using flow-cytometric analysis and compared their profiles to those of neural derivatives. Stem cell and progenitor marker CD133 and epithelial adhesion molecule marker CD326 were more highly expressed in undifferentiated hESCs, whereas neural marker CD56 (NCAM) and neural precursor marker (chemokine receptor) CD184 were more highly expressed in hESC-derived neural cells. CD326 expression levels were consistently higher in all nondifferentiated hESC lines than in neural cell derivatives. In addition, CD326-positive hESCs produced teratomas in SCID mouse testes, whereas CD362-negative neural populations did not. Thus, CD326 may be useful as a novel marker of undifferentiated hESCs to exclude undifferentiated hESCs from differentiated neural cell, populations prior to transplantation. (C) 2008 Elsevier B.V. All rights reserved.