Transgenic labeling of higher order neuronal circuits linked to phospholipase C-β2-expressing taste bud cells in medaka fish.
Transgenic labeling of higher order neuronal circuits linked to phospholipase C-β2-expressing taste bud cells in medaka fish.
复制标题
青鳉鱼中与表达磷脂酶 C-β2 的味蕾细胞相关的高级神经元回路的转基因标记。
DOI：
10.1002/cne.23256
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发表时间：
2013
影响因子：
2.5
通讯作者：
家木誉史
中科院分区：
文献类型：
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作者：
Yutaka Taketani;Masashi Masuda;Hisami Yamanaka-Okumura;Sawako Tatsumi;Hiroko Segawa;Ken-ichi Miyamoto;Eiji Takeda;Hironori Yamamoto;家木誉史
味觉在脊椎动物的食物选择行为中起着关键作用。我们已经证明，磷脂酶C基因（plc-β2）的鱼类直系同源物在味蕾细胞亚群中表达，该味蕾细胞将味觉刺激传递到中枢神经系统，以唤起有利和厌恶的行为。我们在青鳉β 2基因调控区的控制下产生了表达麦胚凝集素（WGA）的转基因青鳉，以分析连接到这些感觉细胞的神经回路。受精后12天的转基因鱼的免疫组织化学分析显示，WGA蛋白被转移到颅感觉神经节和几个核在后脑。在3月龄转基因鱼的后脑中的次级味觉核中也检测到WGA信号。在9月龄转基因鱼的几个间脑和端脑区域观察到WGA信号。标记的大脑区域的年龄依赖性增加强烈表明标记发生在味蕾细胞，并逐渐扩展到中枢神经系统的颅神经和神经元。这些数据是第一次证明了与味蕾细胞的特定亚群相关的高阶味觉神经元回路的追踪。这些结果提供了深入了解的基本神经结构的味觉信息处理是常见的脊椎动物。神经学比较杂志521：1781-1802，2013。© 2012 Wiley Periodicals，Inc.
The sense of taste plays a pivotal role in the food‐selecting behaviors of vertebrates. We have shown that the fish ortholog of the phospholipase C gene (plc‐β2) is expressed in a subpopulation of taste bud cells that transmit taste stimuli to the central nervous system to evoke favorable and aversive behaviors. We generated transgenic medaka expressing wheat germ agglutinin (WGA) under the control of a regulatory region of the medakaplc‐β2gene to analyze the neuronal circuit connected to these sensory cells. Immunohistochemical analysis of the transgenic fish 12 days post fertilization revealed that the WGA protein was transferred to cranial sensory ganglia and several nuclei in the hindbrain. WGA signals were also detected in the secondary gustatory nucleus in the hindbrain of 3‐month‐old transgenic fish. WGA signals were observed in several diencephalic and telencephalic regions in 9‐month‐old transgenic fish. The age‐dependent increase in the labeled brain regions strongly suggests that labeling occurred at taste bud cells and progressively extended to cranial nerves and neurons in the central nervous system. These data are the first to demonstrate the tracing of higher order gustatory neuronal circuitry that is associated with a specific subpopulation of taste bud cells. These results provide insight into the basic neuronal architecture of gustatory information processing that is common among vertebrates. J. Comp. Neurol. 521:1781–1802, 2013. © 2012 Wiley Periodicals, Inc.