Characterization and PCR multiplexing of polymorphic microsatellite loci for the invasive ant Wasmannia auropunctata
Characterization and PCR multiplexing of polymorphic microsatellite loci for the invasive ant Wasmannia auropunctata
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DOI：
10.1111/j.1471-8286.2005.00889.x
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发表时间：
2005-06-01
期刊：
影响因子：
--
通讯作者：
Estoup, A
中科院分区：
文献类型：
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作者：
Fournier, D;Foucaud, J;Estoup, A
高度多态性的遗传标记为昆虫社会性进化研究中遗传参数的估计提供了一个有用的工具。我们分离和表征了12个多态性微卫星位点的入侵蚂蚁，Wasmannia auropunctata，并描述了实验条件的PCR（聚合酶链反应）多重和同时基因分型这些位点在两组5和7个标记。每个位点的等位基因数从2到14不等，观察到的杂合度从0.233到0.967不等。此外，跨物种扩增试验的结果报告在其他三个物种的Wasmannia和两个物种的属Allomerus。
Highly polymorphic genetic markers provide a useful tool for estimating genetic parameters in studies of the evolution of sociality in insects. We isolated and characterized 12 polymorphic microsatellite loci in the invasive ant, Wasmannia auropunctata, and described experimental conditions for PCR (polymerase chain reaction) multiplexing and simultaneously genotyping these loci in two sets of five and seven markers. The number of alleles per locus ranged from two to 14 and the observed heterozygosity ranged from 0.233 to 0.967. Moreover, results of cross-species amplification tests are reported in three other species of Wasmannia and in two species of the genus Allomerus.