Ectopic expression of BABY BOOM triggers a conversion from vegetative to embryonic growth
Ectopic expression of BABY BOOM triggers a conversion from vegetative to embryonic growth
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DOI：
10.1105/tpc.001941
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发表时间：
2002-08-01
期刊：
影响因子：
11.6
通讯作者：
Campagne, MMV
中科院分区：
文献类型：
--
作者：
Boutilier, K;Offringa, R;Campagne, MMV
植物胚胎途径的启动和维持的分子机制在很大程度上是未知的。为了更深入地了解这些过程，我们使用消减杂交来鉴定在油菜未成熟花粉粒体外诱导胚胎发育（小孢子胚胎发生）过程中上调的基因。其中一个基因BABY BOOM（BBM）与AP 2/ERF家族的转录因子相似，并且优先在发育中的胚胎和种子中表达。BBM在拟南芥和芸苔属植物中的异位表达导致了体细胞胚和子叶样结构的自发形成。异位BBM表达诱导额外的多效性表型，包括肿瘤生长，外植体的无瘤再生，以及叶和花形态的改变。BBM在发育种子中的表达模式与BBM过表达表型相结合，表明该基因在胚胎发生过程中促进细胞增殖和形态发生中的作用。
The molecular mechanisms underlying the initiation and maintenance of the embryonic pathway in plants are largely unknown. To obtain more insight into these processes, we used subtractive hybridization to identify genes that are upregulated during the in vitro induction of embryo development from immature pollen grains of Brassica napus (microspore embryogenesis). One of the genes identified, BABY BOOM (BBM), shows similarity to the AP2/ERF family of transcription factors and is expressed preferentially in developing embryos and seeds. Ectopic expression of BBM in Arabidopsis and Brassica led to the spontaneous formation of somatic embryos and cotyledon-like structures on seedlings. Ectopic BBM expression induced additional pleiotropic phenotypes, including neoplastic growth, hormone-free regeneration of explants, and alterations in leaf and flower morphology. The expression pattern of BBM in developing seeds combined with the BBM overexpression phenotype suggests a role for this gene in promoting cell proliferation and morphogenesis during embryogenesis.