Evaluation of a newly developed down-flow immunoassay for detection of serum mannan antigens in patients with candidaemia.
Evaluation of a newly developed down-flow immunoassay for detection of serum mannan antigens in patients with candidaemia.
复制标题
评估新开发的下流式免疫测定法，用于检测念珠菌血症患者的血清甘露聚糖抗原。
DOI：
10.1099/jmm.0.46314-0
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发表时间：
2006
影响因子：
3
通讯作者：
T. Hashimoto
中科院分区：
文献类型：
--
作者：
S. Fujita;T. Takamura;M. Nagahara;T. Hashimoto
已经开发了一种下流免疫测定法来检测血清甘露聚糖抗原，该试验最近作为Unimedi Candida monotest上市。使用105例念珠菌血症患者的251份血清样本，对Unimedi念珠菌monotest与Cand-Tec乳胶凝集试验和2种微板酶免疫试验（Platelia Candida Ag试验和Unimedi Candida）进行了比较。175例无真菌感染和肺红细胞增多临床和微生物学证据的发热患者作为对照组进行检查。Cand-Tec试验的敏感性为38%，特异性为82%。念珠菌血小板抗原检测、Unimedi念珠菌检测和Unimedi念珠菌monotest检测的敏感性和特异性分别为53和92%、69和89%以及82和96%。Unimedi念珠菌单项试验诊断近平滑念珠菌引起的念珠菌血症的敏感性显著高于Plateria Candida Ag试验（P<0.01）。β-D-葡聚糖测定具有95%的高灵敏度和84%的特异性。在74例血清在血培养阳性前或血培养阳性时可用的念珠菌血症患者中，分别有29例（39%）、38例（51%）和48例（65%）患者使用血小板念珠菌抗原试验、Unimedi念珠菌试验和Unimedi念珠菌monotest检测到抗原血症。Unimedi念珠菌monotest似乎是早期诊断侵袭性念珠菌病的一个有前途的工具，因为该测试敏感，简单，快速（约10分钟）。1、成本效益高。
A down-flow immunoassay has been developed to detect serum mannan antigens, and the test was recently marketed as the Unimedi Candida monotest. Using 251 serum samples from 105 patients with candidaemia, a comparison of the Unimedi Candida monotest with the Cand-Tec latex agglutination test and 2 microplate enzyme immunoassay tests (Platelia Candida Ag test and Unimedi Candida) was conducted. One hundred and seventy-five febrile patients without clinical and microbiological evidence of fungal infections and pneumocytosis were examined as controls. The Cand-Tec test had a sensitivity of 38% and a specificity of 82%. The sensitivity and specificity of the Platelia Candida Ag test, the Unimedi Candida and the Unimedi Candida monotest were 53 and 92%, 69 and 89% and 82 and 96%, respectively. The sensitivity of the Unimedi Candida monotest was significantly (P<0.01) higher than that of the Plateria Candida Ag test for diagnosing candidaemia caused by Candida parapsilosis. The beta-D-glucan assay had a high sensitivity of 95%, with a specificity of 84%. Of 74 patients with candidaemia whose sera were available before or on positive blood culture sampling, 29 (39%), 38 (51%) and 48 (65%) patients had antigenemia detected using the Platelia Candida Ag test, the Unimedi Candida and the Unimedi Candida monotest, respectively. The Unimedi Candida monotest seems to be a promising tool for the early diagnosis of invasive candidiasis, because the test was sensitive, simple, rapid (approx. 1 h) and cost-effective.