Expression of TRPV1 in rabbits and consuming hot pepper affects its body weight
Expression of TRPV1 in rabbits and consuming hot pepper affects its body weight
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家兔TRPV1表达及食用辣椒对其体重的影响
DOI：
10.1007/s11033-012-1592-1
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发表时间：
2012-07-01
影响因子：
2.8
通讯作者：
Liu, Enqi
中科院分区：
文献类型：
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作者：
Yu, Qi;Wang, Yanli;Liu, Enqi
辣椒素受体是一种重要的膜受体，被称为瞬时受体潜在香草素亚家族成员1(TRPV1)，与肥胖、糖尿病、代谢综合征和心血管疾病有关。兔模型被认为是研究心血管和代谢性疾病的理想模型，然而，在该模型中，TRPV1的组织表达及其配体辣椒素在饮食诱导肥胖中的生理功能尚未完全确定。在本研究中，我们利用实时定量RT-PCR和Western印迹分析研究了TRPV1在正常兔体内的组织表达。兔TRPV1mRNA在肾脏、肾上腺、脾和脑等多个器官中高表达。系统进化分析表明，兔TRPV1的氨基酸序列比啮齿动物的TRPV1更接近人的TRPV1。为了研究辣椒素(辣椒中的一种刺激性化合物)对体重的影响，给兔子喂以高脂饲料(作为对照)和含有1%辣椒的高脂肪饲料。我们发现，喂食辣椒的兔的体重明显低于对照组。结论：摄入辣椒素可预防饮食诱导的肥胖，兔模型可用于研究TRPV1在心血管和代谢性疾病中的功能。
The capsaicin receptor, known as transient receptor potential vanilloid subfamily member 1 (TRPV1), is an important membrane receptor that has been implicated in obesity, diabetes, metabolic syndrome and cardiovascular diseases. The rabbit model is considered excellent for studying cardiovascular and metabolic diseases, however, the tissue expression of TRPV1 and physiological functions of its ligand capsaicin on diet-induced obesity have not been fully defined in this model. In the current study, we investigated the tissue expression of TRPV1 in normal rabbits using real-time RT-PCR and Western blot analysis. Rabbit TRPV1 mRNA was highly expressed in a variety of organs, including the kidneys, adrenal gland, spleen and brain. A phylogenetic analysis showed that the amino acid sequence of rabbit TRPV1 was closer to human TRPV1 than rodent TRPV1. To examine the effect of capsaicin (a pungent compound in hot pepper) on body weight, rabbits were fed with either a high fat diet (as control) or high fat diet containing 1% hot pepper. We found that the body weight of the hot pepper-fed rabbits was significantly lower than the control group. We conclude that the intake of capsaicin can prevent diet-induced obesity and rabbit model is useful for the study of TRPV1 function in cardiovascular and metabolic diseases.