STRUCTURAL AND MECHANICAL ASSESSMENT OF DEVELOPING CHICK TENDON
STRUCTURAL AND MECHANICAL ASSESSMENT OF DEVELOPING CHICK TENDON
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DOI：
10.1016/0141-8130(88)90048-7
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发表时间：
1988-08-01
影响因子：
8.2
通讯作者：
SILVER, FH
中科院分区：
文献类型：
--
作者：
MCBRIDE, DJ;TRELSTAD, RL;SILVER, FH
用宏观应力-应变特征的变化来评估鸡腱形态发生过程中肌腱微观结构的变化。分别观察了受精后第14天（第40期）、第16天（第42期）、第17天（第43期）、第21天（第0期）、第23天（第H2期）五个发育阶段。应力-应变曲线的形状、极限抗拉强度和破坏应变被用来跟踪发育过程中生物力学性能的变化。极限抗拉强度从第40期的2.05 MPa （1 MPa = 106 N/m2）提高到孵化后2 d的58.05。在42期和43期之间观察到有统计学意义的抗拉强度增加。40期为13%，42期为22%，43期、H0期和H2期为29%。通过测定羟基脯氨酸含量来评估肌腱胶原蛋白的变化。羟脯氨酸/g干重在形态发生的各个阶段均呈上升趋势，40期为39.17 mg/g， H2期为83.41 mg/g。同期干重从12.91%增加到20.19%。胶原纤维的平均直径约为45 ~ 62 nm。原纤维分布在所有阶段均为单峰分布，但在H2阶段由紧单峰分布（40、42期）变为宽单峰分布。
Changes in macroscopic stress-strain characteristics were used to assess alterations in tendon microstructure during chick tendon morphogenesis. Five periods of chick development were observed: post fertilization days 14 (stage 40), 16 (stage 42), 17 (stage 43), 21 (stage H0), 23 (stage H2). The shapes of the stress-strain curves, ultimate tensile strength, and strain to failure were used to follow changes in the biomechanical properties during development. The ultimate tensile strength increased from 2.05 MPa (1 MPa = 106 N/m2) at stage 40 to 58.05 at 2 days post-hatching. A statistically significant increase in measured tensile strength was observed between stage 42 and stage 43. Strain to failure was 13% at stage 40, 22% at stage 42, and 29% for stages 43, H0, and H2. Changes in teotal tendon collagen were assessed by measuring hydroxyproline content. Hydroxyproline/g of dry weight increased at each successive stage of morphogenesis and ranged from 39.17 mg/g dry weight at stage 40 to 83.41 mg/g dry weight at stage H2. Dry weight increased from 12.91% to 20.19% over the same period. The mean collagen fibril diameter ranged from approximately 45 nm to 62 nm. The fibril distribution was unimodal at all stages, but changed from a tight unimodal (stage 40, 42) to a broad unimodal distribution by stage H2.