Biochemical and physiological validation of a corticosteroid radioimmunoassay for plasma and fecal samples in oldfield mice (Peromyscus polionotus)
Biochemical and physiological validation of a corticosteroid radioimmunoassay for plasma and fecal samples in oldfield mice (Peromyscus polionotus)
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DOI：
10.1016/j.physbeh.2003.09.006
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发表时间：
2003-11-01
影响因子：
2.9
通讯作者：
Lynch, JW
中科院分区：
文献类型：
--
作者：
Good, T;Khan, MZ;Lynch, JW
粪便类固醇的测量为评估圈养和自由生活动物种群的繁殖、环境应激和攻击性提供了一种日益重要的非侵入性技术。在本文中，我们验证了皮质酮(CORT)I-125放射免疫法(ICN PharmPharmticals)对一种小型啮齿动物--小儿麻痹症小鼠的血浆和粪便样本的检测。生化验证表明，该方法能准确测定血浆中皮质醇浓度和粪便中皮质类固醇浓度。生理学验证表明：(1)在扰乱动物笼子的3分钟内采集的血液样本代表“基线”皮质醇浓度，(2)在24小时内收集的粪便皮质类固醇浓度与大约4小时前的血浆皮质醇浓度密切相关。这些结果表明，血浆皮质醇和粪便皮质类固醇检测具有足够的灵敏度，可以检测到Oldfield小鼠体内有生物学意义的皮质类固醇浓度的变化。(C)2003 Elsevier Inc.保留所有权利。
The measurement of fecal steroids provides an increasingly important noninvasive technique for assessing reproduction, environmental stress, and aggression in populations of captive and free-living animals. In this paper, we validated the corticosterone (CORT) I-125-radioimmunoassay (ICN Pharmaceuticals) for plasma and fecal samples in a small rodent species, the oldfield mouse (Peromyscus polionotus subgriseus). The biochemical validations indicated that the assays accurately measured CORT concentrations in the plasma and corticosteroid concentrations in the feces. Physiological validation demonstrated that: (1) blood samples collected within 3 min of disturbing an animal's cage represented "baseline" CORT concentrations, and (2) fecal corticosteroid concentrations collected over a 24-h period closely tracked plasma CORT concentrations approximately 4 h earlier. These results demonstrate that the plasma CORT and fecal corticosteroid assays are sensitive enough to detect biologically meaningful alterations in corticosteroid concentrations in oldfield mice. (C) 2003 Elsevier Inc. All rights reserved.