INSITU OPTICAL HISTOCHEMISTRY OF HUMAN ARTERY USING NEAR-INFRARED FOURIER-TRANSFORM RAMAN-SPECTROSCOPY
INSITU OPTICAL HISTOCHEMISTRY OF HUMAN ARTERY USING NEAR-INFRARED FOURIER-TRANSFORM RAMAN-SPECTROSCOPY
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DOI：
10.1073/pnas.89.8.3473
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发表时间：
1992-04-15
影响因子：
11.1
通讯作者：
RAVA, RP
中科院分区：
文献类型：
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作者：
BARAGA, JJ;FELD, MS;RAVA, RP
在本文中，我们证明近红外傅里叶变换拉曼光谱提供了有关人类主动脉粥样硬化程度的前所未有的生化信息。特别是，弹性蛋白、胶原蛋白、胆固醇、胆固醇酯、脂质、类胡萝卜素和磷灰石沉积物都可以通过使用该技术来识别，从而可以研究疾病过程的每个阶段。此外，这些部分可以在组织受辐射表面下方超过 1.5 毫米处被检测到，可能允许提取有关动脉粥样硬化斑块组织学的三维信息。我们建议该技术可用于动脉粥样硬化（特别是动脉粥样硬化）和一般人类疾病的原位光学组织化学分析。
In this paper we demonstrate that near infrared Fourier transform Raman spectroscopy provides unprecedented biochemical information about the extent of atherosclerosis in human aorta. In particular, elastin, collagen, cholesterol, cholesterol esters, lipids, carotenoids, and calcium apatite deposits all can be discerned by using this technique, permitting study of each stage in the disease process. Additionally, these moieties can be detected over 1.5 mm below the irradiated surface of the tissue, possibly allowing extraction of three-dimensional information about the histology of atherosclerotic plaques. We propose that this technique may be utilized for in situ optical histochemical analysis of atherosclerosis in particular and human disease in general.