ACCUMULATION OF A BRAZIL NUT ALBUMIN IN SEEDS OF TRANSGENIC CANOLA RESULTS IN ENHANCED LEVELS OF SEED PROTEIN METHIONINE
ACCUMULATION OF A BRAZIL NUT ALBUMIN IN SEEDS OF TRANSGENIC CANOLA RESULTS IN ENHANCED LEVELS OF SEED PROTEIN METHIONINE
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DOI：
10.1007/bf00034952
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发表时间：
1992-01-01
影响因子：
5.1
通讯作者：
TOWNSEND, J
中科院分区：
文献类型：
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作者：
ALTENBACH, SB;KUO, CC;TOWNSEND, J
我们通过在转基因植物的种子中表达编码来自巴西坚果的富含蛋氨酸的种子蛋白的嵌合基因，增加了商业冬季品种双低油菜籽的种子蛋白的蛋氨酸含量。转基因双低油菜种子积累了富含蛋氨酸的异源蛋白质，其水平占种子总蛋白的 1.7% 至 4.0%，并且含有高达 33% 的蛋氨酸。富含蛋氨酸的蛋白质的前体在种子中被正确加工，导致 2S 蛋白质部分中出现成熟蛋白质。富含2S甲硫氨酸的蛋白质与双低油菜籽11S种子蛋白质在发育的同时在转基因种子中积累，并在种子发芽时迅速消失。双低油菜籽蛋白中蛋氨酸的增加应该会增加用于动物饲料配方中的双低油菜粕的价值。
We have increased the methionine content of the seed proteins of a commercial winter variety of canola by expressing a chimeric gene encoding a methionine-rich seed protein from Brazil nut in the seeds of transgenic plants. Transgenic canola seeds accumulate the heterologous methionine-rich protein at levels which range from 1.7 % to 4.0 % of the total seed protein and contain up to 33 % more methionine. The precursor of the methionine-rich protein is processed correctly in the seeds, resulting in the appearance of the mature protein in the 2S protein fraction. The 2S methionine-rich protein accumulates in the transgenic seeds at the same time in development as the canola 11S seed proteins and disappears rapidly upon germination of the seed. The increase in methionine in the canola seed proteins should increase the value of canola meal which is used in animal feed formulations.