Effects of APOE rs429358, rs7412 and GSTM1/GSTT1 Polymorphism on Plasma and Erythrocyte Antioxidant Parameters and Cognition in Old Chinese Adults.
Effects of APOE rs429358, rs7412 and GSTM1/GSTT1 Polymorphism on Plasma and Erythrocyte Antioxidant Parameters and Cognition in Old Chinese Adults.
复制标题
APOE rs429358、rs7412和GSTM1/GSTT1多态性对中国老年人血浆和红细胞抗氧化参数及认知的影响
DOI：
10.3390/nu7105391
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发表时间：
2015-09-24
期刊：
影响因子：
5.9
通讯作者：
Xiao R
中科院分区：
文献类型：
--
作者：
Yuan L;Liu J;Dong L;Cai C;Wang S;Wang B;Xiao R
载脂蛋白E(APOE)和氧化损伤与阿尔茨海默病(AD)的风险相关。谷胱甘肽S转移酶基因多态性与机体抗氧化能力有关，并参与氧化损伤相关的慢性病。为探讨载脂蛋白E基因rs429358、rs7412和GSTM1/T1基因多态性对中国老年人抗氧化参数和认知功能的影响，对477名55~75岁的中国成年人进行了社区横断面研究。通过问卷调查，收集了参与者的人口学和生活方式信息。认知功能通过蒙特利尔认知评估(MoCA)测试进行测量。采集空腹静脉血进行APOE rs429358、rs7412和GSTM1/T1基因分型，并进行参数测量。未发现APOE基因rs7412、rs429358和GSTM1/T1基因多态性与认知功能的相关性。APOE rs429358、rs7412基因多态性主要与血浆α-生育酚、低密度脂蛋白胆固醇和血浆总抗氧化能力水平相关。检测了APOE rs429358和GSTT1基因对血浆甘油三酯(TG)水平、红细胞过氧化氢酶(CAT)和GST酶活性的交互作用(p<；0.05)。ApoE rs429358T/C+C/C和GSTT1GSTT1GST基因携带者的血浆甘油三酯水平、红细胞过氧化氢酶活力和谷胱甘肽转氨酶活力均显著高于其他基因携带者(P<0.05)；−基因携带者的血浆甘油三酯水平、红细胞过氧化氢酶活力和谷胱甘肽转氨酶活力最低。APOE rs7412T/C+T/T和GSTM1+基因携带者的红细胞过氧化氢酶活性最低，谷胱甘肽过氧化物酶活性最高。血浆和红细胞抗氧化指标水平与APOE基因相关。GSTM1或GSTT1基因改变了APOE rs7412、rs429358基因多态对抗氧化指标的影响。
Apolipoprotein E (APOE) and oxidative damage were correlated with the risk of Alzheimer’s disease (AD). Glutathione S-transferase (GST) polymorphism was proved to be associated with body antioxidant capacity and involved in the oxidative damage related chronic diseases. To explore the combined effects of APOE rs429358, rs7412 and GSTM1/T1 polymorphism on antioxidant parameters and cognition in old Chinese adults, a community-based cross-sectional study was carried out in 477 Chinese adults aged from 55 to 75. Information on demography and lifestyle of the participants was collected with a questionnaire. Cognitive function was measured by using a Montreal Cognitive Assessment (MoCA) test. Fasting venous blood samples were collected for APOE rs429358, rs7412 and GSTM1/T1 genotyping, and parameter measurement. No association of APOE rs7412, rs429358 and GSTM1/T1 polymorphisms with cognition was detected in the old Chinese adults. APOE rs429358, rs7412 polymorphism was mainly associated with plasma α-tocopherol, low density lipoprotein cholesterol (LDL-C) and plasma total antioxidant capacity (T-AOC) levels (p < 0.05). Interaction of APOE rs429358 and GSTT1 genotype on the plasma triglyceride (TG) level and erythrocyte catalase (CAT) and GST enzyme activities were detected (p < 0.05). The subjects with APOE rs429358 T/C + C/C and GSTT1− genotype were found to have the highest plasma TG level, erythrocyte CAT enzyme activity, and the lowest GST enzyme activity compared to subjects with other genotypes (p < 0.05). Lowest erythrocyte CAT enzyme activity and highest glutathione peroxidase (GSH-Px) enzyme activity were detected in the subjects with APOE rs7412 T/C + T/T and GSTM1+ genotype as compared with subjects with other genotypes. The levels of plasma and erythrocyte antioxidant parameters were APOE genotype associated. GSTM1 or GSTT1 genotype modified the influence of APOE rs7412, rs429358 polymorphism on antioxidant parameters.