Serum Levels of CK18 M30 and Leptin Are Useful Predictors of Steatohepatitis and Fibrosis in Paediatric NAFLD
Serum Levels of CK18 M30 and Leptin Are Useful Predictors of Steatohepatitis and Fibrosis in Paediatric NAFLD
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DOI：
10.1097/mpg.0b013e3181e376be
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发表时间：
2010-10-01
影响因子：
2.9
通讯作者：
Dhawan, Anil
中科院分区：
文献类型：
--
作者：
Fitzpatrick, Emer;Mitry, Ragai R.;Dhawan, Anil
背景：随着儿童非酒精性脂肪性肝病（NAFLD）患病率的惊人增长，需要采用非侵入性方法对疾病严重程度进行分层。我们的目的是评估血清生物标志物的组合，作为儿科 NAFLD 疾病活动的衡量标准。 患者和方法：纳入了 45 名经活检证实患有 NAFLD 的儿童。使用酶联免疫吸附测定法测量血清中半胱天冬酶裂解的 CK18 片段 (CK18 M30)、透明质酸、瘦素和脂联素，并使用比色测定法测量高灵敏度 C 反应蛋白。结果：中位年龄为 12.7 岁（55% 男孩）。中位体重指数 z 评分为 1.7。 NAFLD 患者的 CK18 M30 水平显着高于对照组，中位数为 288 IU/L 对比 172 IU/L (P < 0.001)，而在脂肪性肝炎患者中，中位数 347 IU/L 对比单纯脂肪变性（NAFLD 活动评分 = F2）可以与无/轻微纤维化区分开来（
Background: With the alarming growth in prevalence of paediatric nonalcoholic fatty liver disease (NAFLD), there is a need for noninvasive methods of stratifying disease severity. Our aim was to evaluate a combination of serum biomarkers as a measure of disease activity in paediatric NAFLD.Patients and Methods: Forty-five children with biopsy-proven NAFLD were enrolled. Caspase-cleaved CK18 fragments (CK18 M30), hyaluronic acid, leptin, and adiponectin were measured in serum using enzyme-linked immunosorbent assays and high-sensitivity C-reactive protein using a colorimetric assay.Results: Median age was 12.7 years (55% boys). Median body mass index z score was 1.7. CK18 M30 levels were significantly higher in patients with NAFLD versus controls, median 288 IU/L versus 172 IU/L (P < 0.001), and in those with steatohepatitis, median 347 IU/L versus simple steatosis (NAFLD activity score = F2) could be differentiated from no/minimal fibrosis (