Acellular hemoglobin solution enters compressed lung capillaries more readily than red blood cells.
Acellular hemoglobin solution enters compressed lung capillaries more readily than red blood cells.
复制标题
无细胞血红蛋白溶液比红细胞更容易进入压缩的肺毛细血管。
DOI：
10.1152/jappl.2000.89.3.1198
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Harms,BA
中科院分区：
文献类型：
--
作者：
Conhaim,RL;Rodenkirch,LA;Watson,KE;Harms,BA
高肺膨胀压力压迫肺泡间隔毛细血管，阻碍红细胞运输，干扰氧合。然而，最近引入的非细胞血红蛋白溶液可能比红细胞更容易进入被压缩的肺毛细血管。为了验证这一假设，我们用荧光标记的双阿司匹林交联血红蛋白（DCLHb; 10%）和/或自体红细胞灌注离体大鼠肺（红细胞比容，20）。肺充气压力高于血管压力（区1），导致间隔毛细血管受压。共聚焦显微镜检查显示DCLHb分布于肺泡间隔。此外，这种分布不受向灌注液中加入红细胞的影响。我们估计间隔内最大的非细胞血红蛋白质量相当于15个红细胞。相比之下，我们发现1区平均每隔隔2.7±4.6个红细胞。在2区和3区，这些数值分别增加到30.4±25.8和50.4±22.1个细胞/隔。我们得出结论，与红细胞灌注相比，在1区灌注10%的脱细胞血红蛋白溶液可使每隔血红蛋白浓度增加多达五倍。
High lung inflation pressures compress alveolar septal capillaries, impede red cell transit, and interfere with oxygenation. However, recently introduced acellular hemoglobin solutions may enter compressed lung capillaries more easily than red blood cells. To test this hypothesis, we perfused isolated rat lungs with fluorescently labeled diaspirin cross-linked hemoglobin (DCLHb; 10%) and/ or autologous red cells (hematocrit, 20). Septal capillaries were compressed by setting lung inflation pressure above vascular pressures (zone 1). Examination by confocal microscopy showed that DCLHb was distributed throughout alveolar septa. Furthermore, this distribution was not affected by adding red blood cells to the perfusate. We estimated the maximum acellular hemoglobin mass within septa to be equivalent to that of 15 red blood cells. By comparison, we found an average of 2.7 ± 4.6 red cells per septum in zone 1. These values increased to 30.4 ± 25.8 and 50.4 ± 22.1 cells per septum in zones 2 and 3, respectively. We conclude that perfusion in zone 1 with a 10% acellular hemoglobin solution may increase the hemoglobin concentration per septum up to fivefold compared with red cell perfusion.