Kinetics of inactivation of Bacillus spores using low temperature argon plasma at different microwave power densities
Kinetics of inactivation of Bacillus spores using low temperature argon plasma at different microwave power densities
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不同微波功率密度下低温氩等离子体灭活芽孢杆菌孢子的动力学
DOI：
10.1002/1521-3846(200111)21:4
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
I. Hayakawa
中科院分区：
文献类型：
--
作者：
D. Purevdorj;N. Igura;M. Shimoda;O. Ariyada;I. Hayakawa
本研究的目的是确定在聚丙烯生物指示剂载体上沉积的枯草芽孢杆菌、嗜热脂肪芽孢杆菌和短小芽孢杆菌的微波功率密度在灭活中的显著作用。特别地，用Ar等离子体测定的孢子存活率是作为生物指示剂的初始孢子密度的函数。在温度为15℃、气压为50Pa时，在微波功率密度为1.47、2.63和4.21W/cm3的条件下，产生了低温分布分别为31、35和43℃的微波感生Ar等离子体。结果表明，不同芽胞对Ar等离子体表现出不同程度的敏感性，随着等离子体微波功率密度的增加，芽胞存活率显著降低。在三株芽孢杆菌中，枯草芽孢杆菌对Ar等离子体的抗性最强，而嗜热脂肪芽孢杆菌对Ar最敏感。但孢子存活率不受生物指示剂初始孢子密度的影响。尽管短小芽孢杆菌生物指示剂的初始孢子密度有4个数量级的差异，但只观察到一定程度的孢子灭活日志(No/N)，从1.67到1.95。
The objective of this study was to determine the remarkable role of the microwave power density of argon plasma in the inactivation of Bacillus subtilis, Bacillus stearothermophilus and Bacillus pumilus spores deposited on polypropylene bio-indicator carriers. In particular, spore survival by argon plasma was determined as a function of the initial spore density of the bio-indicators. The microwave induced argon plasmas were generated at 1.47, 2.63 and 4.21 w/cm 3 microwave power densities under a low gas pressure of 50 Pa at an ambient temperature of 15 °C to reach low temperature distribution of 31, 35 and 43 °C, respectively. Our results indicate that the different Bacillus spores showed distinct degrees of argon plasma sensitivity, and spore survival was significantly reduced when the microwave power density of the plasma treatments was increased. Among the three Bacillus strains, Bacillus subtilis was the most argon plasma resistant, whereas Bacillus stearothermophilus was the most sensitive. However, spore survival was not affected by the initial spore density of the bio-indicators. Only a certain degree of the spore inactivation log (No/N) from 1.67 to 1.95 was observed despite the 4-order differences in the initial spore density of the Bacillus pumilus bio-indicators.