SIMILAR RESPONSIVENESS OF SMOOTH-MUSCLE OF THE CANINE BASILAR ARTERY TO EDRF AND NITRIC-OXIDE
SIMILAR RESPONSIVENESS OF SMOOTH-MUSCLE OF THE CANINE BASILAR ARTERY TO EDRF AND NITRIC-OXIDE
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DOI：
10.1152/ajpheart.1989.257.4.h1235
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发表时间：
1989-10-01
影响因子：
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通讯作者：
VANHOUTTE, PM
中科院分区：
文献类型：
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作者：
KATUSIC, ZS;MARSHALL, JJ;VANHOUTTE, PM
实验旨在比较犬基底动脉对内皮源性舒张因子（EDRF）和一氧化氮的反应性。使用由缓激肽激活的内皮制剂来研究 EDRF 诱导的松弛，而在不含内皮的制剂中研究一氧化氮的抑制作用。所有实验均在吲哚美辛存在下进行。 EDRF 和一氧化氮诱导的松弛在超氧化物歧化酶加过氧化氢酶存在下显着增强，但在亚甲蓝、LY 83583 和血红蛋白存在下减弱。 M & B 22984 不影响 EDRF 或一氧化氮的松弛。这些结果表明，在犬基底动脉中，EDRF不是氧衍生自由基。基底动脉对 EDRF 和一氧化氮的相似反应性与犬基底动脉中一氧化氮是影响因素的提议一致。
Experiments were designed to compare the reactivity of canine basilar arteries to endothelium-derived relaxing factor (EDRF) and nitric oxide. Preparations with endothelium activated by bradykinin were used to study relaxations induced with EDRF, whereas the inhibitory effect of nitric oxide was studied in preparations without endothelium. All experiments were performed in the presence of indomethacin. EDRF- and nitric oxide-induced relaxations were significantly augmented in the presence of superoxide dismutase plus catalase but were reduced in the presence of methylene blue, LY 83583, and hemoglobin. M & B 22984 did not affect relaxations to either EDRF or nitric oxide. These results indicate that in the canine basilar artery EDRF is not an oxygen-derived free radical. The similar responsiveness of the basilar artery to EDRF and nitric oxide is consistent with the proposal that in the canine basilar artery nitric oxide is the factor.