Neurotensin stimulates neutrophil adherence to bronchial epithelial cells in vitro.
Neurotensin stimulates neutrophil adherence to bronchial epithelial cells in vitro.
复制标题
DOI：
10.1016/0024-3205(95)00088-7
复制
发表时间：
1995-03
期刊：
影响因子：
6.1
通讯作者：
R. Robbins;K. J. Nelson;G. Gossman;I. Rubinstein
中科院分区：
文献类型：
--
作者：
R. Robbins;K. J. Nelson;G. Gossman;I. Rubinstein
嗜中性粒细胞和神经肽都与气道炎症有关。我们假设神经降压素，一种在气道中发现的神经肽，会刺激中性粒细胞粘附到支气管上皮细胞。使用51Cr标记的人中性粒细胞加入到牛支气管上皮细胞的融合单层中来评估粘附性。将神经降压素添加到支气管上皮细胞中产生了时间和浓度依赖性的粘附增加，在4 h和10 − 10 M时达到最大值（17.6 ± 1.4% vs. 6.1 ± 0.4%，p <0.01）。相反，刺激中性粒细胞的神经降压素诱导了浓度依赖性和快速（<5分钟）的粘附增加，在10 − 10 M时也达到最大（27.1 ± 1.9% vs. 10.1 ± 1.4%，p <0.01）。分子的羧基部分再现了这种效应。抗-CD11 a、-CD11b或-ICAM-1抗体显著降低神经降压素诱导的粘附增加。这些数据表明神经降压素在调节气道炎症中的重要作用。
Neutrophils and neuropeptides have both been implicated in airway inflammation. We hypothesized that neurotensin, a neuropeptide found in the airways, would stimulate neutrophil adherence to bronchial epithelial cells. Adherence was assessed using51Cr-labelled human neutrophils added to confluent monolayers of bovine bronchial epithelial cells. Neurotensin added to bronchial epithelial cells produced a time-and concentration-dependent increase in adherence which was maximal at 4 h and 10−10M (17.6 ± 1.4% vs. 6.1 ± 0.4%, p < 0.01). Conversely, neurotensin stimulation of neutrophils induced a concentration-dependent and rapid (< 5 min) increase in adherence which was also maximal at 10−10M (27.1 ± 1.9% vs. 10.1 ± 1.4%, p < 0.01). The effects were reproduced by the carboxy portion of the molecule. Anti-CD11 a, -CD11b or -ICAM-1 antibodies significantly decreased the neurotensin-induced increase in adherence. These data suggest an important role for neurotensin in modulating airway inflammation.