Tissue-Scale Mechanical Coupling Reduces Morphogenetic Noise to Ensure Precision during Epithelial Folding
Tissue-Scale Mechanical Coupling Reduces Morphogenetic Noise to Ensure Precision during Epithelial Folding
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DOI：
10.1016/j.devcel.2020.02.012
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发表时间：
2020-04-20
影响因子：
11.8
通讯作者：
Wang, Yu-Chiun
中科院分区：
文献类型：
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作者：
Eritano, Anthony S.;Bromley, Claire L.;Wang, Yu-Chiun
形态恒定性在发展中的系统中是普遍的。目前尚不清楚精确的形态发生是否源于对基因表达模式的忠实机械解释。我们研究了头沟的形成，这是一种精确定位的线性形态的上皮褶皱。折叠起始由具有单细胞行分辨率的精确遗传密码指定。这种位置编码激活并在空间上限制肌球蛋白的横向收缩性以诱导折叠。然而，20%的起始细胞被错误指定，因为波动的肌球蛋白强度在细胞水平。尽管如此，沟仍然保持线性对齐。我们发现外侧肌球蛋白是平面极化的，将收缩膜界面整合成超细胞带。使用光遗传学在“带”上局部减少机械耦合会降低沟的线性。此外，3D顶点建模表明，极化，相互关联的收缩性赋予形态鲁棒性对噪声。因此，组织尺度的机械耦合作为一种去噪机制，以确保形态发生的精度，尽管有噪声解码的位置信息。
Morphological constancy is universal in developing systems. It is unclear whether precise morphogenesis stems from faithful mechanical interpretation of gene expression patterns. We investigate the formation of the cephalic furrow, an epithelial fold that is precisely positioned with a linear morphology. Fold initiation is specified by a precise genetic code with single-cell row resolution. This positional code activates and spatially confines lateral myosin contractility to induce folding. However, 20% of initiating cells are mis-specified because of fluctuating myosin intensities at the cellular level. Nevertheless, the furrow remains linearly aligned. We find that lateral myosin is planar polarized, integrating contractile membrane interfaces into supracellular "ribbons." Local reduction of mechanical coupling at the "ribbons" using optogenetics decreases furrow linearity. Furthermore, 3D vertex modeling indicates that polarized, interconnected contractility confers morphological robustness against noise. Thus, tissue-scale mechanical coupling functions as a denoising mechanism to ensure morphogenetic precision despite noisy decoding of positional information.