Histomorphometric evaluation of the effect of doxycycline on the healing of bone defects in experimental diabetes mellitus:: A pilot study
Histomorphometric evaluation of the effect of doxycycline on the healing of bone defects in experimental diabetes mellitus:: A pilot study
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DOI：
10.1053/joms.2002.33859
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发表时间：
2002-08-01
影响因子：
1.9
通讯作者：
Karasu, Ç
中科院分区：
文献类型：
--
作者：
Alkan, A;Erdem, E;Karasu, Ç
目的：糖尿病患者的骨愈合受损，尤其是由于胶原蛋白分解增加。最近，四环素类药物已被用于治疗实验性骨缺损，因为它们具有抗胶原溶解特性，并且已经获得了对愈合过程的积极影响。本研究的目的是开发一种计算机辅助的组织形态计量技术，定量测定再生骨的量在实验性骨缺损的糖尿病啮齿动物model.Materials和方法：本研究探讨了全身性多西环素管理的影响，胫骨骨缺损的愈合健康的白化病大鼠和实验诱导的糖尿病大鼠。将24只雌性白化病大鼠分为4组：糖尿病组、糖尿病加多西环素组、对照组或对照加多西环素组。术后10天和30天对标准化骨缺损进行组织形态计量学检查。使用Zeiss Vision图像分析程序KS 400（Kontron Elektron GmbH，埃兴，德国）进行新骨形成量的组织形态计量学分析。结果：愈合10天时，糖尿病组在缺损内新骨形成量方面与对照组相比愈合较差。然而，多西环素给药对糖尿病组和对照组的影响没有统计学差异。在30天的愈合，有任何groups.Conclusions的新形成的骨的量之间没有统计学上的显着差异：本研究发现，强力霉素管理没有显着改变控制和糖尿病大鼠骨缺损愈合过程中的新骨形成的量。(C)2002年美国口腔颌面外科医师协会。
Purpose: Bone healing is impaired in diabetes mellitus, particularly due to increased collagen breakdown. Recently, tetracyclines have been used to treat experimental bone defects because they have anticollagenolytic properties, and positive effects on the healing process have been obtained. The objective of this study was to develop a computer-assisted histomorphometric technique to quantitatively determine the amount of regenerating bone within experimental bone defects in a diabetic rodent model.Materials and Methods: This study examined the effects of systemic doxycycline administration on the healing of tibial bone defects in healthy albino rats and in experimentally induced diabetic rats. Twenty-four female albino rats were assigned to 4 groups: diabetic, diabetic plus doxycycline, control, or control plus doxycycline. The standardized bone defects were histomorphometrically examined 10 and 30 days postoperatively. Histomorphometric analysis of the amount of new bone formation was performed using the Zeiss Vision image analysis program KS 400 (Kontron Elektron GmbH, Eching, Germany).Results: At 10 days of healing, the diabetic groups exhibited inferior healing compared with the control groups in terms of the amount of new bone formation within the defects. However, the effect of doxycycline administration to the diabetic and control groups was not statistically different. At 30 days of healing, there were no statistically significant differences between the amount of newly formed bone in any of the groups.Conclusions: This study found that doxycycline administration did not significantly alter the amount of new bone formation during the healing of bone defects in control and diabetic rats.(C) 2002 American Association of Oral and Maxillofacial Surgeons.