Novel Marker Gene for Assessment of Behavior of Transgenic Plants in the Field
Novel Marker Gene for Assessment of Behavior of Transgenic Plants in the Field
复制标题
用于评估转基因植物田间行为的新标记基因
DOI：
10.5511/plantbiotechnology.20.225
复制
发表时间：
2003
影响因子：
1.6
通讯作者：
H. Saji
中科院分区：
文献类型：
--
作者：
M. Tamaoki;Yumio Toda;N. Nakajima;A. Kubo;M. Aono;H. Saji
转基因作物的广泛使用引起了一些人的担忧，担心它们会逃逸到田间，并将转基因转移到意想不到的宿主身上。因此，对田间的转基因植物进行跟踪是十分必要的。本研究表明，由knottted1型同源盒基因引起的叶片形态改变可能是一个有用的标记。我们检测了表达同源盒基因的转基因烟草，这些烟草表现出“弯曲”、“褶皱”或“矮化”表型，其叶片畸形很容易用肉眼与野生型植物区分开来。同源盒基因的表达对寄主植物的一些生长参数有不同的影响。然而，“弯曲”型转基因植株的生长与野生型植株没有太大差异，这表明“弯曲”表型是评估田间转基因/转化体去向的有用标记。
The wide use of transgenic crops has raised some concern about their escape into the field and movement of transgene to unintended hosts. Thus, it is essential to keep track of the transgenic plants in the field. Here, we show that alteration of leaf morphology, which is caused by knotted1-type homeobox genes can be a useful marker. We examined transgenic tobacco expressing the homeobox gene that exhibited ‘curved’, ‘wrinkled’, or ‘dwarf’ phenotype whose foliar malformations were easily distinguished from wild-type plants with the naked eye. Expression of the homeobox genes could have various effects on some growth parameters of the host plants. However, the growth of ‘curved’-type transgenic plants was not much different from that of wild-type plants, suggesting that the ‘curved’ phenotype is a useful marker for assessing the whereabouts of transgenes/transformants in the field.