A COMPARISON OF FLUORESCENCE METHODS USED IN THE PHARMACOKINETIC STUDIES OF Zn(II)PHTHALOCYANINE IN MICE
A COMPARISON OF FLUORESCENCE METHODS USED IN THE PHARMACOKINETIC STUDIES OF Zn(II)PHTHALOCYANINE IN MICE
复制标题
酞菁锌(II)在小鼠体内药代动力学研究中荧光方法的比较
DOI：
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发表时间：
1991
影响因子：
3.3
通讯作者：
G. Weagle
中科院分区：
文献类型：
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作者：
R. Biolo;G. Jori;J. Kennedy;P. Nadeau;R. Pottier;E. Reddi;G. Weagle
摘要-通过两种体外技术和一种体内技术（均基于荧光光谱法）监测了封装在二棕榈酰磷脂酰胆碱（DPPC）脂质体中的锌酞菁（ZnPc）在Skh：HR-1裸鼠体内静脉注射的药代动力学。体外方法包括对薄组织切片或这些组织的提取物进行荧光测量。体内方法涉及皮肤表面的荧光测量。这两种体外技术得到了类似的结果，这是与以前的研究结果相一致的ZnPc的药代动力学行为。肝脏和脾脏显示ZnPc浓度快速增加，在给药后30分钟或更短时间内达到最大水平。在皮肤、脂肪或肌肉中检测到相对较少的ZnPc，最大浓度出现在12小时。体内荧光达到最大强度约。注射后6 h，在胸部中部分析部位和12 h，在大腿中。在两个不同的解剖部位的体内测量显示药代动力学行为，其反映了源自几个组织部位的整体整合荧光。
Abstract— The pharmacokinetics of Zn phthalocyanine (ZnPc) encapsulated in dipalmitoyl‐phosphatidyl‐choline (DPPC) liposomes, injected intravenously in Skh:HR‐1 nude mice, was monitored by two in vitro techniques and one in vivo technique, all based on fluorescence spectroscopy. The in vitro methods involve either fluorescence measurements on thin tissue sections or on extracts from these tissues. The in vivo method involves the fluorescence measurement at the skin surface. Both in vitro techniques gave similar results which are consistent with previous findings on the pharmacokinetic behavior of ZnPc. The liver and spleen showed rapid ZnPc concentration increases, reaching a maximum level in 30 min. or less post drug administration. Relatively little ZnPc was detected in the skin, fat or muscle, the maximum concentration occurring at 12h. In vivo fluorescence reached a maximum intensity approx. 6 h post injection at the mid‐chest analysis site and at 12 h in the thigh. The in vivo measurements at two different anatomical sites showed pharmacokinetic behavior that reflects an overall integrated fluorescence originating from several tissue sites.