iTRAQ-based quantitative proteomic analysis of Thermobifida fusca reveals metabolic pathways of cellulose utilization
iTRAQ-based quantitative proteomic analysis of Thermobifida fusca reveals metabolic pathways of cellulose utilization
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DOI：
10.1016/j.jprot.2011.05.038
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发表时间：
2011-09-06
影响因子：
3.3
通讯作者：
Sze, Siu Kwan
中科院分区：
文献类型：
--
作者：
Adav, Sunil S.;Ng, Chee Sheng;Sze, Siu Kwan
棕色热裂菌是一种在加热的有机材料中发现的好氧、嗜热、纤维素降解细菌。本研究应用iTRAQ定量蛋白质组学分析技术对T. fusca在存在和不存在纤维素的情况下生长，以阐明纤维素营养诱导的细胞过程。使用基于iTRAQ的定量蛋白质组学方法，用蛋白质组学方法对纤维素水解过程中表达的783种胞质和181种膜蛋白进行定量。
Thermobifida fusca is an aerobic, thermophilic, cellulose degrading bacterium identified in heated organic materials. This study applied iTRAQ quantitative proteomic analysis to the cellular and membrane proteomes of T. fusca grown in presence and absence of cellulose to elucidate the cellular processes induced by cellulose nutrient. Using an iTRAQ-based quantitative proteomic approach, 783 cytosolic and 181 membrane proteins expressed during cellulose hydrolysis were quantified with