Prevalence of Porphyromonas gingivalis and periodontal health status
Prevalence of Porphyromonas gingivalis and periodontal health status
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DOI：
10.1128/jcm.36.11.3239-3242.1998
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发表时间：
1998-11-01
影响因子：
9.4
通讯作者：
Leys, EJ
中科院分区：
文献类型：
--
作者：
Griffen, AL;Becker, MR;Leys, EJ
牙周炎是一种常见的，渐进的疾病，最终影响到大多数人口。牙周炎的局部破坏被认为是由龈沟的细菌感染引起的，并且一些临床研究已经提供了涉及牙龈卟啉单胞菌的证据。如果牙龈卟啉单胞菌是牙周病原体，则预期其存在于大多数患有疾病的受试者中并且很少在具有良好牙周健康的受试者中检测到。牙龈卟啉单胞菌在大多数患病的受试者中未被检测到，并且年龄匹配的牙周健康对照未被包括用于比较。本文报道的研究的目的是比较牙龈卟啉单胞菌在牙周炎组与牙周健康组中的患病率，一个全面的采样策略和敏感的PCR检测被用来最大限度地提高检测的可能性。牙龈卟啉单胞菌特异性扩增的靶序列是核糖体操纵子内的转录间隔区。牙龈卟啉单胞菌在健康受试者中的检出率仅为25%（46/181），而在牙周炎组中的检出率为79%（103/130）（P < 0.0001）。牙周炎组牙龈卟啉单胞菌感染的优势比是健康组的11.2倍（95%可信区间为6.5 ~ 19.2）。这些数据表明牙龈卟啉单胞菌在牙周炎的发病机制中起作用，并提示牙龈卟啉单胞菌可能不是牙周健康牙列的正常居民。
Periodontitis is a common, progressive disease that eventually affects the majority of the population. The local destruction of periodontitis is believed to result from a bacterial infection of the gingival sulcus, and several clinical studies have provided evidence to implicate Porphyromonas gingivalis, If P, gingivalis is a periodontal pathogen, it would be expected to be present in most subjects with disease and rarely detected in subjects with good periodontal health, However, in most previous studies, P, gingivalis has not been detected in the majority of subjects with disease, and age-matched, periodontally healthy controls were not included for comparison, The purpose of the study reported here aas to compare the prevalence of P, gingivalis in a group with periodontitis to that of a group that is periodontally healthy, A comprehensive sampling strategy and a sensitive PCR assay were used to maximize the likelihood of detection. The target sequence for P, gingivalis-specific amplification was the transcribed spacer region within the ribosomal operon, P. gingivalis was detected in only 25% (46 of 181) of the healthy subjects but was detected in 79% (103 of 130) of the periodontitis group (P < 0.0001). The odds ratio for being infected with P. gingivalis was 11.2 times greater in the periodontitis group than in the healthy group (95% confidence interval, 6.5 to 19.2), These data implicate P, gingivalis in the pathogenesis of periodontitis and suggest that P. gingivalis may not be a normal inhabitant of a periodontally healthy dentition.