Continuous Uroflow Cystometry in the Urethane-Anesthetized Mouse
Continuous Uroflow Cystometry in the Urethane-Anesthetized Mouse
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DOI：
10.1002/nau.20850
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发表时间：
2010-01-01
影响因子：
2
通讯作者：
Kuchel, George A.
中科院分区：
文献类型：
--
作者：
Smith, Phillip P.;Kuchel, George A.
目的：体内动物膀胱测压代表了下尿路生理学研究的公认方法。小鼠模型的一个特殊优势是转基因品系的可用性，提供了将个体基因与相关生理事件联系起来的可能性。然而，由于排空期间液滴形成和释放不连续，小排空体积使通过重量法获得可靠的压力流量数据的能力变得复杂。我们研究了在乌拉坦麻醉下膀胱测压期间简单的非重力连续尿液收集系统的可行性，并将乌拉坦麻醉与清醒膀胱测压进行比较。方法：使用耻骨上管对清醒且乌拉坦麻醉的雌性小鼠进行膀胱测压。在聚氨酯麻醉的动物中使用了一种简单、新颖的非重量分析尿液收集方法，以评估排尿量并允许计算流速。比较组间压力和时间相关变量。结果：在此模型中，无效尿液收集似乎是完整且连续的。平均排尿量为0.09+/-0.020ml，平均流速为0.029+/-0.007ml/秒。聚氨酯麻醉延迟膀胱测压/容量反应。然而，排尿反射完好无损，并且各组之间具有可比性。雌性小鼠通过先前在大鼠中描述的脉动加压来排尿。结论：在乌拉坦麻醉下使用简单可靠的尿液收集方法进行耻骨上排尿膀胱测量在小鼠中是可行的，允许将排尿量与压力和时间数据整合。体积和流量数据的包含增强了小鼠模型在逼尿肌和潜在括约肌性能的体内评估中的有用性。神经尿素。尿动力学。 29:1344-1349, 2010。(C) 2010 Wiley-Liss, Inc.
Purpose: In vivo animal cystometry represents an accepted methodology for the study of lower urinary tract physiology. A particular advantage of the mouse model is the availability of genetically modified strains, offering the possibility of linking individual genes to relevant physiological events. However, small voided volumes complicate the ability to obtain reliable pressure-flow data by gravimetric methods, due to non-continuous drop formation and release during voiding. We investigated the feasibility of a simple non-gravimetric continuous urine collection system during cystometry under urethane anesthesia, and compared urethane-anesthetized with awake cystometry. Methods: Cystometry was performed in awake and urethane-anesthetized female mice using a suprapubic tube. A simple, novel non-gravimetric method of urine collection was used in urethane-anesthetized animals to assess voided volume and permit flow rate calculations. Pressure and time-related variables were compared between groups. Results: Voided urine collection appears to be complete and continuous in this model. Mean voided volume was 0.09 +/- 0.020 ml, with an average flow rate of 0.029 +/- 0.007 ml/sec. Urethane anesthesia delayed cystometric pressure/volume responses. However, micturition reflexes were intact and otherwise comparable between groups. Female mice void with pulsatile pressurization previously described in rats. Conclusion: Suprapubic voiding cystometry using a simple and reliable urine collection method under urethane anesthesia is feasible in mice, permitting the integration of voided volumes with pressure and time data. The inclusion of volume and flow data enhances the usefulness of the mouse model for in vivo assessment of detrusor and potentially sphincteric performance. Neurourol. Urodynam. 29:1344-1349, 2010. (C) 2010 Wiley-Liss, Inc.