Genetic interactions between mutants of the 'error-prone' repair group of Saccharomyces cerevisiae and their effect on recombination and mutagenesis
Genetic interactions between mutants of the 'error-prone' repair group of Saccharomyces cerevisiae and their effect on recombination and mutagenesis
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DOI：
10.1016/s0921-8777(97)00070-0
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发表时间：
1998-03-01
期刊：
影响因子：
--
通讯作者：
Kupiec, M
中科院分区：
文献类型：
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作者：
Liefshitz, B;Steinlauf, R;Kupiec, M
我们已经创建了一个等基因系列的酵母菌株携带遗传系统，以监测不同类型的重组和突变[B。Liefshitz，A.帕尔凯特河Maya，M.库皮茨DNA修复基因在酵母中重复序列之间重组中的作用，遗传学140（1995）1199-1211.]。在本研究中，我们的特点的影响基因的“易错”或postreplicative修复组的重组和突变。我们发现，拉德5和拉德18菌株有自发重组水平升高，异位基因转换和直接重复序列之间的重组。重组水平的增加在两种突变体和rad 5 rad 18双突变体中是相似的，表明RAD 5和RAD 18基因产物一起作用于自发重组。相反，RAD 5和RAD 18在诱变修复中发挥替代作用：这些基因中的每一个中的突变都提高了CAN 1位点的自发正向突变，但是当两个基因都缺失时，观察到低水平的自发诱变。突变修复的RAD 5/RAD 18途径依赖于REV 3编码的跨损伤聚合酶。我们分析了RAD 5和RAD 18基因产物与其他修复基因之间的相互作用。在rad 5和rad 18突变体中观察到的高重组水平依赖于RAD 1、RAD 51、RAD 52和RAD 57基因。Srs 2解旋酶在产生由RAD 5和RAD 18基因产物加工的重组发生底物中起重要作用。(C)1998年Elsevier Science B.V.
We have created an isogenic series of yeast strains that carry genetic systems to monitor different types of recombination and mutation [B. Liefshitz, A. Parket, R. Maya, M. Kupiec. The role of DNA repair genes in recombination between repeated sequences in yeast, Genetics 140 (1995) 1199-1211.]. In the present study we characterize the effect of mutations in genes of the 'error-prone' or postreplicative repair group on recombination and mutation. We show that rad5 and rad18 strains have elevated levels of spontaneous recombination, both of ectopic gene conversion and of recombination between direct repeats. The increase in recombination levels is similar in both mutants and in the rad5 rad18 double mutant, suggesting that the RAD5 and RAD18 gene products act together with respect to spontaneous recombination. In contrast, RAD5 and RAD18 play alternative roles in mutagenic repair: mutations in each of these genes elevate spontaneous forward mutation at the CAN1 locus, but when both genes are deleted, a low level of spontaneous mutagenesis is seen. The RAD5/RAD18 pathway of mutagenic repair is dependent on the REV3-encoded translesion polymerase. We analyze the interactions between the RAD5 and RAD18 gene products and other repair genes. The high recombination levels seen in rad5 and rad18 mutants is dependent on the RAD1, RAD51, RAD52, and RAD57 genes. The Srs2 helicase plays an important role in creating the recombinogenic substrate(s) processed by the RAD5 and RAD18 gene products. (C) 1998 Elsevier Science B.V.