Design and synthesis of dual-excitation fluorescent probe, Tb3+-dtpa-bis(fluorescein), and application in detection of hydrazine in environmental water samples and live cells
Design and synthesis of dual-excitation fluorescent probe, Tb3+-dtpa-bis(fluorescein), and application in detection of hydrazine in environmental water samples and live cells
复制标题
双激发荧光探针Tb3-dtpa-bis(荧光素)的设计与合成及其在环境水样和活细胞中肼检测中的应用
DOI：
10.1016/j.dyepig.2018.10.044
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发表时间：
2019-03-01
影响因子：
4.5
通讯作者：
Song, Youtao
中科院分区：
文献类型：
--
作者：
Li, Xinyi;Yu, Zhiyue;Song, Youtao
基于Tb 3 +-dtpa-BHz和荧光素在247 nm和480 nm激发波长下分别在515 nm和520 nm处的特殊发射，设计了一种检测肼的新型双激发荧光探针Tb 3 +-dtpa-bis（fluorescein）。考察了肼浓度、溶液pH值、干扰物质等因素对肼测定的影响。Tb ~（3+）-dtpa-bis（fluorescein）检测肼的灵敏度和特异性均较高。Tb ~（3+）-dtpa-bis（fluorescein）在515 nm和520 nm处的荧光发射强度分别与肼浓度在3.00 × 10 ~（-5）mol L ~（-1）~ 4.50 × 10 ~（-5）mol L ~（-1）范围内呈良好的线性关系。247 nm波长光激发的LOD为13 ppb（4.08 × 10（-7）mol L-1），480 nm波长光激发的LOD为8 ppb（2.50 × 10（-7）mol L-1），分别接近和低于EPA的TLV（10 ppb）。基于密度泛函理论（DFT）对探针及其最终产物的HOMO-LUMO能级和分子轨道界面图进行了理论计算。此外，还对真实的水样和活细胞中肼的检测进行了研究。实验结果表明，该荧光探针可用于环境水样和活细胞中肼的测定。
A novel dual-excitation fluorescent probe, Tb3+-dtpa-bis(fluorescein), for detecting hydrazine is designed based on the special emissions of Tb3+-dtpa-BHz and fluorescein at 515 nm and 520 nm, respectively, under 247 nm and 480 nm excitation wavelength lights. The affecting factors including hydrazine concentration, solution pH value and interfering substances on the detection of hydrazine are examined. The high sensitivity and high specificity of Tb3+-dtpa-bis(fluorescein) in hydrazine detection could be confirmed. Two good linear responses of fluorescence emission intensities of Tb3+-dtpa-bis(fluorescein) at 515 nm and 520 nm as functions of hydrazine concentration in range of 3.00 x 10(-5) mol L-1-4.50 x 10(-5) mol L-1 are obtained, respectively. The LOD of 13 ppb (4.08 x 10(-7) mol L-1) for 247 nm wavelength light excitation and 8 ppb (2.50 x 10(-7) mol L-1) for 480 nm wavelength light excitation are achieved, which are close to and lower than TLV (10 ppb) of the EPA, respectively. The theoretical calculations on HOMO-LUMO energy levels and the interfacial plots of the molecular orbitals for probe and its final product based on density functional theory (DFT) are performed. In addition, the detections of hydrazine in real water samples and living cells are also studied. The experimental results indicated that the developed fluorescent probe could be applied for the determination of hydrazine in environmental water samples and living cells.