Formation of Ni- and Zn-Sulfides in Cultures of Sulfate-Reducing Bacteria
Formation of Ni- and Zn-Sulfides in Cultures of Sulfate-Reducing Bacteria
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DOI：
10.1080/01490450701758239
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发表时间：
2007-12
影响因子：
2.3
通讯作者：
Jonathan P. Gramp;J. Bigham;K. Sasaki;O. Tuovinen
中科院分区：
文献类型：
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作者：
Jonathan P. Gramp;J. Bigham;K. Sasaki;O. Tuovinen
本研究的目的是表征硫酸盐还原细菌培养物中沉淀的镍硫化物和锌硫化物。含有 58 mM SO 4 2− 的无铁培养基用氯化镍和氯化锌进行修正，然后进行接种。在 22°C、45°C 和 60°C 孵育两周后，从培养物中取样。通过在其他相同的条件下使无菌液体培养基与 Na 2 S 溶液反应来制备非生物对照。厌氧收集沉淀物，冷冻干燥，并通过 X 射线衍射 (XRD)、扫描电子显微镜和总 Ni、Zn 和 S 进行分析。在含 Ni 介质中，生物成因硫化物沉淀物主要是黄褐铁矿 (Ni 3 S 2 )，而非生物沉淀物是混合的黑褐铁矿和六角铁矿 (NiS 2 )。生物镍沉淀物比相应的非生物样品具有更好的结晶性。通过 XRD 鉴定了从含锌介质中采集的沉淀物中的闪锌矿 (ZnS)。扫描电子显微镜揭示了硫化物无序的形态特征，其在生物样品中主要以细颗粒的聚集体形式出现，而非生物沉淀物则含有更多的板状和针状结构。
The purpose of this study was to characterize Ni- and Zn-sulfides precipitated in sulfate-reducing bacterial cultures. Fe-free media containing 58 mM SO 4 2− were amended with Ni and Zn chloride followed by inoculation. Precipitates were sampled from cultures after two weeks of incubation at 22, 45, and 60 ° C. Abiotic controls were prepared by reacting bacteria-free liquid media with Na 2 S solutions under otherwise identical conditions. Precipitates were collected anaerobically, freeze-dried and analyzed by x-ray diffraction (XRD), scanning electron microscopy, and for total Ni, Zn, and S. In Ni-containing media, biogenic sulfide precipitates were mostly heazelwoodite (Ni 3 S 2 ), whereas abiotic precipitates were mixed heazelwoodite and vaesite (NiS 2 ). The biogenic Ni-precipitates were better crystalline than the corresponding abiotic samples. Sphalerite (ZnS) was identified by XRD in precipitates sampled from Zn-containing media. Scanning electron microscopy revealed disordered morphological features for the sulfides, which occurred mostly as aggregates of fine particles in biogenic samples, whereas abiotic precipitates contained more plate- and needle-like structures.