INCREASED LEVELS OF PEROXISOMAL ACTIVE OXYGEN-RELATED ENZYMES IN COPPER-TOLERANT PEA-PLANTS
INCREASED LEVELS OF PEROXISOMAL ACTIVE OXYGEN-RELATED ENZYMES IN COPPER-TOLERANT PEA-PLANTS
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DOI：
10.1104/pp.85.2.570
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发表时间：
1987-10-01
期刊：
影响因子：
7.4
通讯作者：
DELRIO, LA
中科院分区：
文献类型：
--
作者：
PALMA, JM;GOMEZ, M;DELRIO, LA
在体内研究了高铜营养水平(240微摩尔)对两个对铜敏感的豌豆品种叶片叶绿体、过氧化物体和线粒体中铜、锰、铁、锌金属酶活性的影响。所研究的金属酶是：线粒体的细胞色素c氧化酶，锰-超氧化物歧化酶和铜，锌-超氧化物歧化酶I，过氧化氢酶和过氧化氢酶，以及叶绿体的铜，锌-超氧化物歧化酶II。耐铜植物和铜敏感植物线粒体超氧化物歧化酶同工酶(Mn-SOD和铜锌超氧化物歧化酶I)的活性非常相似，而细胞色素c氧化酶的活性在铜敏感植物中较低。两个植物品种的叶绿体铜、锌-超氧化物歧化酶活性相同。而铜胁迫下，耐铜植物体内的过氧化物酶活性显著高于铜敏感植物，过氧化氢酶活性也显著高于铜敏感植物。在耐铜植物中，这些过氧化物体活性相关酶的活性较高，表明这些酶参与了活性氧中间产物(O2-，OH.cntdo.)也暗示了过氧化物酶在豌豆植物耐铜的分子机制中的作用。
The effect in vivo of high nutrient levels of copper (240 micromolar) on the activity of different metalloenzymes containing Cu, Mn, Fe, and Zn, distributed in chloroplasts, peroxisomes, and mitochrondria, was studied in leaves of two varieties of Pisum sativum L. plants with different sensitivity to copper. The metalloenzymes studied were: cytochrome c oxidase, Mn-superoxide dismutase (Mn-SOD) and Cu,Zn-superoxide dismutase I (Cu,Zn-SOD I), for mitochondria; catalase and Mn-SOD, for peroxisomes; and isozyme Cu,Zn-SOD II for chloroplasts. The activity of mitochondrial SOD isozymes (Mn-SOD and Cu, Zn-SOD I) was very similar in Cu-tolerant and Cu-sensitive plants, whereas cytochrome c oxidase was lower in Cu-sensitive plants. Chloroplastid Cu,Zn-SOD activity was the same in the two plant varieties. In contrast, the peroxisomal Mn-SOD activity was considerably higher in Cu-tolerant than in Cu-sensitive plants, and the activity of catalase was also increased in peroxisome of Cu-tolerant plants. The higher activities of these peroxisomal active oxygen-related enzymes in Cu-tolerant plants suggest the involvement or reactive oxygen intermediates (O2-, OH.cntdot.) in the mechanism of Cu lethality, and also imply a function for peroxisomal Mn-SOD in the molecular mechanisms of plant tolerance to Cu in Pisum sativum L.