Changing expression profiles of lncRNAs, mRNAs, circRNAs and miRNAs during osteoclastogenesis.
Changing expression profiles of lncRNAs, mRNAs, circRNAs and miRNAs during osteoclastogenesis.
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破骨细胞生成过程中 lncRNA、mRNA、circRNA 和 miRNA 表达谱的变化。
DOI：
10.1038/srep21499
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发表时间：
2016-02-09
影响因子：
4.6
通讯作者：
Dong S
中科院分区：
文献类型：
--
作者：
Dou C;Cao Z;Yang B;Ding N;Hou T;Luo F;Kang F;Li J;Yang X;Jiang H;Xiang J;Quan H;Xu J;Dong S
骨是一个动态的器官，不断地经历着塑形、修复和重塑。骨的稳态是通过成骨细胞骨形成和骨吸收之间的平衡来维持的。破骨细胞（Osteoclasts，OC）是来源于造血干细胞（hematopoietic stem cells，HSC）或单核细胞/巨噬细胞祖细胞的特化多核细胞。破骨细胞的形成经历了不同的阶段，而细胞间融合是形成功能性破骨细胞的重要步骤之一。本研究采用基因芯片技术检测破骨细胞RAW 264. 7细胞在不同阶段lncRNA、mRNA、circRNA和miRNA的表达情况。选择经常变化的RNA，并在四组之间进行Venn分析。结果显示，在OC分化的各个阶段，有518个lncRNA、207个mRNAs、24个circRNA和37个miRNAs的表达经常发生变化。采用基因本体论（GO）和京都基因与基因组百科全书（KEGG）生物学途径分析，预测差异表达的lncRNA和共表达的潜在靶向基因的功能。对差异表达的RNA进行相关性分析，构建lncRNA-mRNA和circRNA-miRNA的共表达网络。本研究对非编码RNA（ncRNA）在破骨细胞发生中的潜在功能提供了系统的观点。
Bone is a dynamic organ continuously undergoing shaping, repairing and remodeling. The homeostasis of bone is maintained by the balance between osteoblastic bone formation and osteoclastic bone resorption. Osteoclasts (OCs) are specialized multinucleated cells derived from hematopoietic stem cells (HSCs) or monocytes/macrophage progenitor cells. There are different stages during osteoclastogenesis, and one of the most important steps to form functional osteoclasts is realized by cell-cell fusion. In our study, microarray was performed to detect the expression profiles of lncRNA, mRNA, circRNA and miRNA at different stages during osteoclastogenesis of RAW264.7 cells. Often changed RNAs were selected and clustered among the four groups with Venn analysis. The results revealed that expressions of 518 lncRNAs, 207 mRNAs, 24 circRNAs and 37 miRNAs were often altered at each stage during OC differentiation. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) biological pathway analysis were performed to predict the functions of differentially expressed lncRNAs and co-expressed potential targeting genes. Co-expression networks of lncRNA-mRNA and circRNA-miRNA were constructed based on the correlation analysis between the differentially expressed RNAs. The present study provided a systematic perspective on the potential function of non-coding RNAs (ncRNAs) during osteoclastogenesis.