Flocked nasal swab versus nasopharyngeal aspirate in adult emergency room patients: similar multiplex PCR respiratory pathogen results and patient discomfort
Flocked nasal swab versus nasopharyngeal aspirate in adult emergency room patients: similar multiplex PCR respiratory pathogen results and patient discomfort
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DOI：
10.3109/23744235.2015.1096956
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发表时间：
2016-03-03
影响因子：
5.8
通讯作者：
Nilsson, Anna C.
中科院分区：
文献类型：
--
作者：
Hansen, Karin B.;Westin, Johan;Nilsson, Anna C.
采用鼻咽抽吸物（NPA）和植绒鼻拭子（fNS）对50例有呼吸道感染症状或急性极度疲劳发作的成人急诊室患者进行采样。在29例临床诊断为呼吸道感染的患者中，20例通过定性流感PCR和18价多重PCR检测呼吸道病原体，在21例未诊断的患者中，3例检测呼吸道病原体。PCR在11例患者的NPA样本和10例患者的fNS样本中检测到甲型和B型流感病毒。当通过NPA采样时，60%的患者感觉到很少或没有不适，当通过fNS采样时，66%的患者感觉到不适。我们的结论是，NPA和fNS是同样敏感的检测呼吸道病原体的多重PCR，两种采样方法没有显着不同的患者感到不适（p=0.171，Wilcoxon符号秩检验）。因此，在某些环境和情况下，通过fNS进行侵入性较小的采样可能是优选的。
Fifty adult emergency room patients with symptoms of respiratory tract infections or acute onset of extreme fatigue were sampled by both nasopharyngeal aspirate (NPA) and flocked nasal swab (fNS). Respiratory agents were detected by a qualitative influenza PCR and an 18-valent multiplex PCR in 20 of 29 patients with a clinical diagnosis of respiratory tract infection, and in 3 of 21 without such a diagnosis. PCR detected influenza A and B in NPA samples from 11 patients and in fNS samples from 10 patients. Little or no discomfort was perceived by 60% of the patients when sampled by NPA and by 66% when sampled by fNS. We conclude that NPA and fNS were equally sensitive for detection of respiratory agents by multiplex PCR, and the two sampling methods did not differ significantly regarding discomfort perceived by patients (p=0.171, Wilcoxon signed rank test). Hence less invasive sampling by fNS might be preferable in certain settings and situations.