Simultaneous separation and purification of five bioactive coumarins from the Chinese medicinal plant Cnidium monnieri by high-speed countercurrent chromatography
Simultaneous separation and purification of five bioactive coumarins from the Chinese medicinal plant Cnidium monnieri by high-speed countercurrent chromatography
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DOI：
10.1002/jssc.200400002
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发表时间：
2005-02-01
影响因子：
3.1
通讯作者：
Chen, F
中科院分区：
文献类型：
--
作者：
Li, HB;Chen, F
蛇床子Cndium monnieri(L.)芦荟是一种著名的中草药，用于治疗阳萎、寒战和皮肤相关疾病，具有很强的止痒、抗过敏、抗皮肤病、抗菌、抗真菌和抗骨质疏松活性。建立了一种从该植物中分离纯化5种生物活性香豆素的高速逆流色谱方法。采用乙醇提取法从蛇床子中提取香豆素类化合物。Cusson正在接受超声波治疗。以正己烷-乙酸乙酯-乙醇-水(5：5：4：6，v/v)和正己烷-乙酸乙酯-乙醇-水(5：5：6：4，v/v)为溶剂，采用分步洗脱的方法成功地完成了高速逆流色谱。这五种相对纯度较高的香豆素是从500毫克的粗提物中一次获得的。其纯度为90.6-98.9%，回收率为85.7-94.2%。
Cnidium monnieri (L.) Cusson is a well-known Chinese medicinal plant, which has been used for the treatment of impotence, frigidity, and skin-related diseases, and exhibits strong antipruritic, antiallergic, antidermatophytic, antibacterial, antifungal, and antiosteoporotic activities. A high-speed counter-current chromatography method was developed for the separation and purification of five bioactive coumarins from this plant. The crude coumarins were obtained by ethanol extraction from the dried fruits of Cnidium monnieri (L.) Cusson under sonication. High-speed counter-current chromatography with the two-phase solvent systems n-hexane-ethyl acetate-ethanol-water (5:5:4:6, v/v) and n-hexane-ethyl acetate-ethanol-water (5:5:6:4, v/v) was successfully performed with stepwise elution. The five relatively pure coumarins were obtained from 500 mg of the crude extract in a single run. Their purities were 90.6-98.9%, and the recoveries were 85.7-94.2%.