Regulation of cell proliferation in rat mandibular condylar cartilage in explant culture by insulin-like growth factor-1 and fibroblast growth factor-2
Regulation of cell proliferation in rat mandibular condylar cartilage in explant culture by insulin-like growth factor-1 and fibroblast growth factor-2
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DOI：
10.1016/s0003-9969(02)00052-3
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发表时间：
2002-09-01
影响因子：
3
通讯作者：
Hinton, RJ
中科院分区：
文献类型：
--
作者：
Fuentes, MA;Opperman, LA;Hinton, RJ
胰岛素样生长因子-1 (IGF-1) 和成纤维细胞生长因子-2 (FGF-2) 调节生长板软骨细胞的增殖和分化，但令人惊讶的是，人们对下颌骨髁突等次级软骨生长调节的潜在机制知之甚少。这里的目的是研究 28 日龄雄性 Sprague-Dawley 大鼠体内下颌髁软骨中是否存在 IGF-1 和 FGF-2 受体（通过免疫组织化学），该软骨的增殖如何对外植体培养物中外源 IGF-1 或 FGF-2 浓度的增加作出反应（通过 [H-3] 胸苷掺入），以及这些生长因子及其受体的表达是否在软骨在青春期过渡期间发生变化（定量逆转录酶聚合酶链反应）。 IGF-1 和 FGF-2 受体 (R)（IGF-1R、FGFR1 和 FGFR3）的免疫反应性在成软骨细胞和肥大软骨细胞中最明显，而 FGFR2 免疫反应性在关节和软骨母细胞前区最强。尽管FGF-2产生显着反应的阈值浓度较低，但外源IGF-1引起的增殖反应明显大于FGF-2诱导的增殖反应。在从青春期前（31天）到青春期后期开始（42天）的过渡期间，IGF-1基因表达增加和FGF-2基因表达减少的明显趋势是明显的。在这些受体中，只有 FGFR2 和 FGFR3 表达增加。这些数据提供的证据表明，下颌髁软骨的增殖可能部分受到IGF-I和FGF-2的调节，并且这些基因的表达在青春期发生显着变化。数据还表明，控制下颌髁软骨增殖的机制可能与调节颅缝的机制和调节生长板的机制有很多共同点。 (C) 2002 Elsevier Science Ltd. 保留所有权利。
Insulin-like growth factor-1 (IGF-1) and fibroblast growth factor-2 (FGF-2) regulate the proliferation and differentiation of growth-plate chondrocytes, but surprisingly little is known of the mechanisms underlying growth regulation in secondary cartilages such as the mandibular condylar. The aims here were to investigate whether IGF-1 and FGF-2 receptors are present in mandibular condylar cartilage in vivo from 28-day-old male Sprague-Dawley rats (by immunohistochemistry), how proliferation in that cartilage responds to increasing concentrations of exogenous IGF-1 or FGF-2 in explant culture (by [H-3]thymidine incorporation), and whether the expression of these growth factors and their receptors in the cartilage changes during the transition to puberty (quantitative reverse transcriptase-polymerase chain reaction). Immunoreactivity for receptors (R) for IGF-1 and FGF-2 (IGF-1R, FGFR1, and FGFR3) was most pronounced in chondroblasts and hypertrophic chondrocytes, while FGFR2 immunoreactivity was strongest in the articular and prechondroblastic zones. The proliferative response elicited by exogenous IGF-1 was considerably greater than that induced by FGF-2, although the threshold concentration for a significant response was lower for FGF-2. In the transition from prepuberty (31 days) to the beginning of late puberty (42 days), a pronounced trend of increasing IGF-1 and decreasing FGF-2 gene expression was evident. Of the receptors, only FGFR2 and FGFR3 expression increased. These data provide evidence that proliferation in the mandibular condylar cartilage might be regulated in part by IGF- I and FGF-2, and that expression of these genes changes considerably at puberty. The data also suggest that mechanisms governing proliferation in mandibular condylar cartilage might have as much in common with those regulating cranial sutures as those regulating growth-plate. (C) 2002 Elsevier Science Ltd. All rights reserved.